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. 1988 Jul;85(14):4953-5.
doi: 10.1073/pnas.85.14.4953.

Catalysis of concerted reactions by antibodies: the Claisen rearrangement

D Hilvert  1 , S H Carpenter, K D Nared, M T Auditor
Affiliations

Catalysis of concerted reactions by antibodies: the Claisen rearrangement

D Hilvert et al. Proc Natl Acad Sci U S A. 1988 Jul.

Abstract

Monoclonal antibodies were prepared against a transition state analog inhibitor of chorismate mutase (EC 5.4.99.5). One of the antibodies catalyzes the rearrangement of chorismate to prephenate with rate accelerations of more than 2 orders of magnitude compared to the uncatalyzed reaction. Saturation kinetics were observed, and at 25 degrees C the values of kcat and Km were 1.2 X 10(-3) s-1 and 5.1 X 10(-5) M respectively. The transition state analog was shown to be a competitive inhibitor of the reaction with Ki equal to 0.6 microM. These results demonstrate the feasibility of using rationally designed immunogens to generate antibodies that catalyze concerted reactions.

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