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. 2021 Apr 23:15:1727-1733.
doi: 10.2147/OPTH.S296116. eCollection 2021.

Activity of Deposited Lysozyme on Contemporary Soft Contact Lenses Exposed to Differing Lens Care Systems

Miriam Heynen  1 , Alan Ng  1 , Elizabeth Martell  1 , Lakshman N Subbaraman  1 , Lyndon Jones  1   2
Affiliations

Activity of Deposited Lysozyme on Contemporary Soft Contact Lenses Exposed to Differing Lens Care Systems

Miriam Heynen et al. Clin Ophthalmol. .

Abstract

Purpose: The amount of protein deposition on soft contact lenses and to what extent the proteins are denatured may have an impact on comfortable wearing times of contact lenses. The purpose of this study was to evaluate the effects of two lens care systems on total protein and the quantity and activity of lysozyme deposited on worn senofilcon A, silicone hydrogel contact lenses.

Participants and methods: Thirty symptomatic soft contact lens wearers were enrolled into a 4-week prospective, randomized, bilateral eye, daily-wear, crossover, double-masked study. Participants were fitted with biweekly senofilcon A lenses and were assigned either a polyquaternium-1 and myristamidopropyl dimethylamine-containing system (OPTI-FREE RepleniSH) or a peroxide-based system (CLEAR CARE). After each wear period, proteins were extracted from the lenses and analyzed for total protein, total lysozyme quantity and activity.

Results: The use of either the peroxide-based system or the polyquaternium-1 and myristamidopropyl dimethylamine-containing system resulted in no difference (P>0.05) to the amount of total protein deposited on the lenses (6.7 ± 2.8 micrograms/lens versus 7.3 ± 2.8 micrograms/lens, respectively) or to the amount of denatured lysozyme deposits (0.8 ± 0.7 versus 0.9 ± 0.7 micrograms/lens), respectively. The total amount of lysozyme deposited on the lenses was significantly lower when using the peroxide-based system (1.3 ± 0.9 micrograms/lens) compared to the polyquaternium-1 and myristamidopropyl dimethylamine-containing system (1.7 ± 1.0 micrograms/lens) (P=0.02).

Conclusion: The inactivation of lysozyme deposited on senofilcon A lenses when disinfected with the peroxide-based or the polyquaternium-1 and myristamidopropyl dimethylamine-containing systems were neither statistically nor clinically significant and the overall amounts of denatured lysozyme recovered from the lenses were low (<1 microgram/lens).

Keywords: contact lens; contact lens care system; lysozyme; protein activity; silicone hydrogel.

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Conflict of interest statement

L Jones is a consultant and/or serves on advisory boards for Alcon, CooperVision, Johnson & Johnson Vision, Novartis and Ophtecs. L Jones also reports grants from Alcon during the conduct of the study. His research group has contracts and/or receives personal fees from Alcon, Allergan, Allied Innovations, Aurinia Pharma, BHVI, CooperVision, GL Chemtech, i-Med Pharma, Johnson & Johnson Vision, Lubris, Menicon, Natures Way, Novartis, Ophtecs, Ote Pharma, PS Therapy, Santen, Shire, SightGlass, SightSage, and Visioneering, outside the submitted work. L Subbaraman and A Ng were employed at CORE while the study was conducted. A Ng is currently employed at Market Lane Optical, Woodbridge, Ontario, Canada and L Subbaraman is currently employed at Alcon Research, LLC, Vision Care Franchise, Johns Creek, Georgia, USA. The authors report no other conflicts of interest in this work.

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