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. 2021 Apr;10(6):e12048.
doi: 10.1002/jev2.12048. Epub 2021 Apr 27.

A call for Rigor and standardization in plant extracellular vesicle research

Marcela Pinedo  1 , Laura de la Canal  1 , Carine de Marcos Lousa  2   3
Affiliations

A call for Rigor and standardization in plant extracellular vesicle research

Marcela Pinedo et al. J Extracell Vesicles. 2021 Apr.
No abstract available

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Figures

FIGURE 1
FIGURE 1
Representation of Plant EV and PDNV isolation procedures. Recovery of apoplastic extracellular fluids is compared with soft tissue rupture. For simplicity, only two types of putative EVs are shown: ectosome‐types (derived from plasma membrane) or exosome‐types (derived from MVBs originating from the Golgi). After differential ultracentrifugation, purification from apoplastic fluids will lead to the purification of natural EVs (exosomes, ectosomes….etc). In contrast, the use of soft tissue rupture will result in “artificial” plant derived nanovesicles (PDNVs) which will contain EVs mixed with intracellular organelle debris such as mitochondria (red), chloroplasts (dark green), ER (yellow), and so forth.
FIGURE 2
FIGURE 2
Protein families frequently recovered in Plant EVs. Proteomes of A. thaliana, Sunflower, Olive and N. benthamiana were compared to retrieve common identities and represent them in a Venn diagram. In red, proteins that are shared between the four proteomes.
See this image and copyright information in PMC

References

    1. An, Q. , Hückelhoven, R. , Kogel, K.‐H. , & van Bel, A. J. E. (2006). Multivesicular bodies participate in a cell wall‐associated defence response in barley leaves attacked by the pathogenic powdery mildew fungus. Cellular Microbiology, 8, 1009–1019. - PubMed
    1. Baldrich, P. , Rutter, B. D. , Karimi, H. Z. , Podicheti, R. , Meyers, B. C. , & Innes, R. W. (2019). Plant extracellular vesicles contain diverse small RNA species and are enriched in 10‐ to 17‐nucleotide “Tiny” RNAs. Plant Cell, 31, 315–324. - PMC - PubMed
    1. Cai, Q. , Qiao, L. , Wang, M. , He, B. , Lin, F.‐M. , Palmquist, J. , Huang, S‐Da , & Jin, H. (2018). Plants send small RNAs in extracellular vesicles to fungal pathogen to silence virulence genes. Science, 360, 1126–1129. - PMC - PubMed
    1. Campbell, K. (2020). Do the microRNAs we eat affect gene expression? Nature, 582, S10–S11.
    1. Childers, D. S. , Avelar, G. M. , Bain, J. M. , Larcombe, D. E. , Pradhan, A. , Budge, S. , Heaney, H. , & Brown, A. J. P. (2020). Impact of the Environment upon the Candida albicans Cell Wall and Resultant Effects upon Immune Surveillance. Curr Top Microbiol Immunol., 425, 297–330). Springer. - PubMed

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