Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Apr 27:16:3041-3057.
doi: 10.2147/IJN.S290254. eCollection 2021.

The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition

Jian Yu #  1 , Luyao Yi #  1 , Rui Guo  1 , Jingmei Guo  1 , Hongye Yang  1 , Cui Huang  1
Affiliations

The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition

Jian Yu et al. Int J Nanomedicine. .

Abstract

Background: The dentin exposure always leads to dentin hypersensitivity and/or caries. Given the dentin's tubular structure and low mineralization degree, reestablishing an effective biobarrier to stably protect dentin remains significantly challenging. This study reports a versatile dentin surface biobarrier consisting of a mesoporous silica-based epigallocatechin-3-gallate (EGCG)/nanohydroxyapatite delivery system and evaluates its stability on the dentinal tubule occlusion and the Streptococcus mutans (S. mutans) biofilm inhibition.

Materials and methods: The mesoporous delivery system was fabricated and characterized. Sensitive dentin discs were prepared and randomly allocated to three groups: 1, control group; 2, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) group; and 3, the mesoporous delivery system group. The dentin permeability, dentinal tubule occlusion, acid and abrasion resistance, and S. mutans biofilm inhibition were determined for 1 week and 1 month. The in vitro release profiles of EGCG, Ca, and P were also monitored.

Results: The mesoporous delivery system held the ability to sustainably release EGCG, Ca, and P and could persistently occlude dentinal tubules with acid and abrasion resistance, reduce the dentin permeability, and inhibit the S. mutans biofilm formation for up to 1 month compared with the two other groups. The system provided prolonged stability to combat oral adverse challenges and served as an effective surface biobarrier to protect the exposed dentin.

Conclusion: The establishment of the dentin surface biobarrier consisting of a mesoporous delivery system indicates a promising strategy for the prevention and the management of dentin hypersensitivity and caries after enamel loss.

Keywords: biofilm; dentin; epigallocatechin-3-gallate; mesoporous silica; nanohydroxyapatite.

PubMed Disclaimer

Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Chemical structure of epigallocatechin-3-gallate (EGCG).
Figure 2
Figure 2
Schematic of the experimental design conducted in the present study.
Figure 3
Figure 3
Ultrastructure and surface characteristics of MSN and EGCG@nHAp@MSN.
Figure 4
Figure 4
Dentin permeability values from each time point (subjected to EDTA etching, surface treatments, acid challenge, or abrasion challenge) for (A and B) 1 week or (C and D) 1 month of storage.
Figure 5
Figure 5
Tubule occlusion from FESEM cross- and longitudinal-section observation after surface treatments.
Figure 6
Figure 6
Tubule occlusion from FESEM cross- and longitudinal-section observation after surface treatments and storage and then subjected to acid challenge.
Figure 7
Figure 7
Tubule occlusion from FESEM cross- and longitudinal-section observation after surface treatments and storage and then subjected to abrasion challenge.
Figure 8
Figure 8
S. mutans biofilm grown on dentin surfaces from CLSM and FESEM images.
Figure 9
Figure 9
S. mutans biofilm grown on dentin surfaces from CFU counts and MTT assay and cytotoxicity of EGCG@nHAp@MSN from CCK-8 assay.
Figure 10
Figure 10
In vitro cumulative release of (A) EGCG and (B) Ca and P of EGCG@nHAp@MSN in sterile deionized water for 30 days at 37 °C.
See this image and copyright information in PMC

References

    1. Shao C, Jin B, Mu Z, et al. Repair of tooth enamel by a biomimetic mineralization frontier ensuring epitaxial growth. Sci Adv. 2019;5(8):eaaw9569. doi:10.1126/sciadv.aaw9569 - DOI - PMC - PubMed
    1. Lawn BR, Lee JJW, Chai H. Teeth: among nature’s most durable biocomposites. Annu Rev Mat Res. 2010;40(1):55–75. doi:10.1146/annurev-matsci-070909-104537 - DOI
    1. An B, Wang R, Zhang D. Role of crystal arrangement on the mechanical performance of enamel. Acta Biomater. 2012;8(10):3784–3793. doi:10.1016/j.actbio.2012.06.026 - DOI - PubMed
    1. Chiang YC, Lin HP, Chang HH, et al. A mesoporous silica biomaterial for dental biomimetic crystallization. ACS Nano. 2014;8(12):12502–12513. doi:10.1021/nn5053487 - DOI - PubMed
    1. Palmer LC, Newcomb CJ, Kaltz SR, Spoerke ED, Stupp SI. Biomimetic systems for hydroxyapatite mineralization inspired by bone and enamel. Chem Rev. 2008;108(11):4754–4783. doi:10.1021/cr8004422 - DOI - PMC - PubMed

MeSH terms

LinkOut - more resources