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Comment
. 2021 Apr 20;2(4):100249.
doi: 10.1016/j.xcrm.2021.100249.

Measuring the size and decay dynamics of the HIV-1 latent reservoir

Runxia Liu  1 , Allison A Catalano  1   2 , Ya-Chi Ho  1
Affiliations
Comment

Measuring the size and decay dynamics of the HIV-1 latent reservoir

Runxia Liu et al. Cell Rep Med. .

Abstract

Measuring HIV-1 latent reservoir is essential for HIV-1 cure strategies. Levy et al.1 developed a multiplex droplet digital PCR (ddPCR) assay-5-target intact proviral DNA assay-to detect multiple regions of HIV-1 proviral genome and increase accuracy.

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Figures

Figure 1
Figure 1
Measuring the size of the HIV-1 latent reservoir and the dynamics of HIV-1 proviral landscape (A) Comparison of inducible RNA-based, proviral sequencing-based, and DNA ddPCR-based methods that measure the size of HIV-1 latent reservoir. (B) Caveats of using HIV-1 near full-length proviral sequencing and multiplex ddPCR to profile the dynamics of intact versus defective HIV-1 proviral landscape. Ψ, packaging signal. RRE, Rev-response element.
See this image and copyright information in PMC

Comment on

  • A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir.
    Levy CN, Hughes SM, Roychoudhury P, Reeves DB, Amstuz C, Zhu H, Huang ML, Wei Y, Bull ME, Cassidy NAJ, McClure J, Frenkel LM, Stone M, Bakkour S, Wonderlich ER, Busch MP, Deeks SG, Schiffer JT, Coombs RW, Lehman DA, Jerome KR, Hladik F. Levy CN, et al. Cell Rep Med. 2021 Apr 12;2(4):100243. doi: 10.1016/j.xcrm.2021.100243. eCollection 2021 Apr 20. Cell Rep Med. 2021. PMID: 33948574 Free PMC article.

References

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