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Review
. 2021:2228:85-116.
doi: 10.1007/978-1-0716-1024-4_8.

Quantitative Mass Spectrometry-Based Proteomics: An Overview

Svitlana Rozanova  1   2 , Katalin Barkovits  1   2 , Miroslav Nikolov  3 , Carla Schmidt  4 , Henning Urlaub  3   5   6 , Katrin Marcus  7   8
Affiliations
Review

Quantitative Mass Spectrometry-Based Proteomics: An Overview

Svitlana Rozanova et al. Methods Mol Biol. 2021.

Abstract

In recent decades, mass spectrometry has moved more than ever before into the front line of protein-centered research. After being established at the qualitative level, the more challenging question of quantification of proteins and peptides using mass spectrometry has become a focus for further development. In this chapter, we discuss and review actual strategies and problems of the methods for the quantitative analysis of peptides, proteins, and finally proteomes by mass spectrometry. The common themes, the differences, and the potential pitfalls of the main approaches are presented in order to provide a survey of the emerging field of quantitative, mass spectrometry-based proteomics.

Keywords: Absolute quantification; Chemical labeling; Isotope labeling; Label-free; Mass spectrometry; Metabolic labeling; Proteomics; Relative quantification.

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