doi: 10.1016/0006-291x(88)90648-1.
Cell-cycle dependent transformation competence in Dictyostelium discoideum
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- PMID: 3395345
- DOI: 10.1016/0006-291x(88)90648-1
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Cell-cycle dependent transformation competence in Dictyostelium discoideum
Biochem Biophys Res Commun.
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Abstract
We describe a modification of the transformation procedure for Dictyostelium which allows for a more exact estimate of transformation efficiency and the isolation of primary transformants. Investigations of transformation competence revealed a negative correlation to cell density and a distinct distribution during the cell-cycle. In synchronized cells, transformation efficiency is 2-3 fold higher during mitosis when compared to unsynchronized cells.
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