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. 1988 Jul 15;154(1):54-9.
doi: 10.1016/0006-291x(88)90648-1.

Cell-cycle dependent transformation competence in Dictyostelium discoideum

W Nellen  1 , U Saur
Affiliations

Cell-cycle dependent transformation competence in Dictyostelium discoideum

W Nellen et al. Biochem Biophys Res Commun. .

Abstract

We describe a modification of the transformation procedure for Dictyostelium which allows for a more exact estimate of transformation efficiency and the isolation of primary transformants. Investigations of transformation competence revealed a negative correlation to cell density and a distinct distribution during the cell-cycle. In synchronized cells, transformation efficiency is 2-3 fold higher during mitosis when compared to unsynchronized cells.

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