Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021:2306:257-273.
doi: 10.1007/978-1-0716-1410-5_17.

Analytical Methodologies for Lipidomics in Hemp Plant

Andrea Cerrato  1 , Anna Laura Capriotti  1 , Carmela Maria Montone  2 , Sara Elsa Aita  1 , Giuseppe Cannazza  3   4 , Cinzia Citti  3   4 , Susy Piovesana  1 , Laganà Aldo  1   4
Affiliations

Analytical Methodologies for Lipidomics in Hemp Plant

Andrea Cerrato et al. Methods Mol Biol. 2021.

Abstract

The chemical composition of Cannabis sativa L. has been extensively studied for tens of years, but little is known about its lipidome. This chapter describes an analytical workflow for polar lipid determination in hemp. After extraction, lipids are enriched and isolated by graphitized carbon black sorbent, and the isolated lipid is analyzed by liquid chromatography (LC) coupled with high resolution mass spectrometry, leading to identification of many lipid species. We have developed a semi-automated platform using commercially available Lipostar software for lipid identification. Our approach affords the identification of 189 polar lipids in hemp extract, including sulfolipids and phospholipids. The number of the identified lipid species is by far the highest ever reported for Cannabis sativa.

Keywords: Cannabis sativa; Graphitized carbon black; Lipidomics; Lipostar; Phospholipids; Sulfolipids.

PubMed Disclaimer

References

    1. Russo EB, Jiang H-E, Li X, Sutton A, Carboni A, del Bianco F, Mandolino G, Potter DJ, Zhao Y-X, Bera S, Zhang Y-B, Lü E-G, Ferguson DK, Hueber F, Zhao L-C, Liu C-J, Wang Y-F, Li C-S (2008) Phytochemical and genetic analyses of ancient cannabis from Central Asia. J Exp Bot 59:4171–4182. https://doi.org/10.1093/jxb/ern260 - DOI - PubMed - PMC
    1. Skoglund G, Nockert M, Holst B (2013) Viking and early middle ages northern Scandinavian textiles proven to be made with hemp. Sci Rep 3:2686. https://doi.org/10.1038/srep02686 - DOI - PubMed - PMC
    1. Small E, Beckstead HD (1973) Common cannabinoid phenotypes in 350 stocks of Cannabis. Lloydia 36:144–165. http://www.ncbi.nlm.nih.gov/pubmed/4744553 - PubMed
    1. Blake A, Nahtigal I (2019) The evolving landscape of cannabis edibles. Curr Opin Food Sci 28:25–31. https://doi.org/10.1016/j.cofs.2019.03.009 - DOI
    1. ElSohly MA, Slade D (2005) Chemical constituents of marijuana: the complex mixture of natural cannabinoids. Life Sci 78:539–548. https://doi.org/10.1016/j.lfs.2005.09.011 - DOI - PubMed

LinkOut - more resources