Exosome loaded genipin crosslinked hydrogel facilitates full thickness cutaneous wound healing in rat animal model
- PMID: 33960253
- PMCID: PMC8118534
- DOI: 10.1080/10717544.2021.1912210
Exosome loaded genipin crosslinked hydrogel facilitates full thickness cutaneous wound healing in rat animal model
Abstract
Full thickness cutaneous wound therapy and regeneration remains a critical challenge in clinical therapeutics. Recent reports have suggested that mesenchymal stem cells exosomes therapy is a promising technology with great potential to efficiently promote tissue regeneration. Multifunctional hydrogel composed of both synthetic materials and natural materials is an effective carrier for exosomes loading. Herein, we constructed a biodegradable, dual-sensitive hydrogel encapsulated human umbilical cord-mesenchymal stem cells (hUCMSCs) derived exosomes to facilitate wound healing and skin regeneration process. The materials characterization, exosomes identification, and in vivo full-thickness cutaneous wound healing effect of the hydrogels were performed and evaluated. The in vivo results demonstrated the exosomes loaded hydrogel had significantly improved wound closure, re-epithelialization rates, collagen deposition in the wound sites. More skin appendages were observed in exosomes loaded hydrogel treated wound, indicating the potential to achieve complete skin regeneration. This study provides a new access for complete cutaneous wound regeneration via a genipin crosslinked dual-sensitive hydrogel loading hUCMSCs derived exosomes.
Keywords: genipin crosslinked hydrogel; hUCMSCs derived exosome; rat model; skin tissue regeneration; wound healing.
Conflict of interest statement
The authors declare no conflict of interest.
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