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. 2021 Jul;9(7):e1701.
doi: 10.1002/mgg3.1701. Epub 2021 May 8.

Blood group genotyping in alloimmunized multi-transfused thalassemia patients from Iran

Reyhaneh Sarihi  1 , Arezoo Oodi  1 , Raziyeh Dadkhah Tehrani  1 , Seyedeh Farzaneh Jalali  1 , Fahimeh Mardani  1 , Azita Azarkeivan  1 , Samira Gudarzi  1 , Naser Amirizadeh  1
Affiliations

Blood group genotyping in alloimmunized multi-transfused thalassemia patients from Iran

Reyhaneh Sarihi et al. Mol Genet Genomic Med. 2021 Jul.

Abstract

Objectives: Serological methods may not be reliable for RBC antigen typing, especially in multi-transfused patients. The blood group systems provoking the most severe transfusion reactions are mainly Rh, Kell, Kidd, and Duffy. We intended to determine the genotype of these blood group system antigens among Iranian alloimmunized thalassemia patients using molecular methods and compare the results with serological phenotyping.

Methods: Two hundred patients participated in this study. Blood group phenotype and genotype were determined using the serological method and PCR-SSP, respectively. The genotypes of patients with incompatibility between phenotype and genotype were re-evaluated by RFLP-PCR and confirmed by DNA sequencing.

Results: Discrepancies between phenotype and genotype results were found in 132 alleles and 83 (41.5%) patients; however, there was complete accordance between the three genotyping methods. Most discrepancies were detected in Rh and Duffy systems with 47 and 45 cases, respectively, and the main discrepancy was in the FY*B/FY*B allele when serologically showed Fy(a+b+). All 39 undetermined phenotypes, due to mixed-field reactions, were resolved by molecular genotyping.

Conclusion: Molecular genotyping is more reliable compared with the serological method, especially in multi-transfused patients. Therefore, the addition of blood group genotyping to serological assays can lead to an antigen-matched transfusion in these patients.

Keywords: alloimmunization; blood groups; genotype; thalassemia.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

References

    1. Arnoni, C. P., Muniz, J. G., Paula, T. A. D., Person, R. D. D. M., Gazito, D., Baleotti, W.Jr., Barreto, J. A., Castilho, L., & Latini, F. R. M. (2013). An easy and efficient strategy for KEL genotyping in a multiethnic population. Revista Brasileira De Hematologia E Hemoterapia, 35(2), 99–102. - PMC - PubMed
    1. Bakanay, S. M., Ozturk, A., Ileri, T., Ince, E., Yavasoglu, S., Akar, N., Uysal, Z., & Arslan, O. (2013). Blood group genotyping in multi‐transfused patients. Transfusion and Apheresis Science, 48(2), 257–261. - PubMed
    1. Belsito, A., Costa, D., Fiorito, C., De Iorio, G., Casamassimi, A., Perrotta, S., & Napoli, C. (2015). Erythrocyte genotyping for transfusion‐dependent patients at the Azienda Universitaria Policlinico of Naples. Transfusion and Apheresis Science, 52(1), 72–77. - PubMed
    1. Belsito, A., Magnussen, K., & Napoli, C. (2017). Emerging strategies of blood group genotyping for patients with hemoglobinopathies. Transfusion and Apheresis Science, 56(2), 206–213. - PubMed
    1. Casas, J., Friedman, D. F., Jackson, T., Vege, S., Westhoff, C. M., & Chou, S. T. (2015). Changing practice: Red blood cell typing by molecular methods for patients with sickle cell disease. Transfusion, 55(6pt2), 1388–1393. - PMC - PubMed

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