Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Mar;7(3):843-9.
doi: 10.1002/j.1460-2075.1988.tb02883.x.

Interaction of a protein from rat liver nuclei with cruciform DNA

M E Bianchi  1
Affiliations

Interaction of a protein from rat liver nuclei with cruciform DNA

M E Bianchi. EMBO J. 1988 Mar.

Abstract

We constructed a synthetic cruciform DNA which closely resembles Holliday junctions, a DNA structure formed during recombination or following the transition from interstrand to intrastrand base pairing in palindromic DNA sequences. We identified and partially purified a protein from rat liver that specifically binds to this cruciform DNA molecule and does not bind to single-stranded or double-stranded DNAs of the same sequence. This protein also binds to the cruciform structure formed by a 70 bp palindromic sequence cloned in plasmid pUC18. No detectable nucleolytic activity is associated with the rat liver cruciform-binding protein, in contrast to all cruciform-recognizing proteins known so far.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3445-9 - PubMed
    1. J Biol Chem. 1987 Sep 15;262(26):12752-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5076-80 - PubMed
    1. J Mol Biol. 1982 Apr 5;156(2):229-43 - PubMed
    1. Cell. 1982 Jun;29(2):357-65 - PubMed

Publication types