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. 1988 Mar;7(3):843-9.
doi: 10.1002/j.1460-2075.1988.tb02883.x.

Interaction of a protein from rat liver nuclei with cruciform DNA

M E Bianchi  1
Affiliations

Interaction of a protein from rat liver nuclei with cruciform DNA

M E Bianchi. EMBO J. 1988 Mar.

Abstract

We constructed a synthetic cruciform DNA which closely resembles Holliday junctions, a DNA structure formed during recombination or following the transition from interstrand to intrastrand base pairing in palindromic DNA sequences. We identified and partially purified a protein from rat liver that specifically binds to this cruciform DNA molecule and does not bind to single-stranded or double-stranded DNAs of the same sequence. This protein also binds to the cruciform structure formed by a 70 bp palindromic sequence cloned in plasmid pUC18. No detectable nucleolytic activity is associated with the rat liver cruciform-binding protein, in contrast to all cruciform-recognizing proteins known so far.

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