Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 May 8;6(1):180.
doi: 10.1038/s41392-021-00574-8.

SARS-CoV-2 promote autophagy to suppress type I interferon response

Xianfeng Hui #  1 , Linliang Zhang #  2 , Lei Cao  1 , Kun Huang  1 , Ya Zhao  1 , Yufei Zhang  1 , Xi Chen  1 , Xian Lin  3 , Mingzhou Chen  4 , Meilin Jin  5
Affiliations

SARS-CoV-2 promote autophagy to suppress type I interferon response

Xianfeng Hui et al. Signal Transduct Target Ther. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
SARS-CoV-2 promote autophagy to suppress type I interferon response. a GFP-LC3 dot formation in Vero-E6 cells transiently transfected with GFP-LC3 and either left uninfected (Mock) or infected with SARS-CoV-2 (MOI of 0.05) for 48 h or treated with CQ for 4 h. Scale bar, 10 µm. b Vero-E6 were uninfected (−) or infected (+) with SARS-CoV-2. Lysates were evaluated by western blotting (WB). c EM analysis of Vero-E6 cells that were stimulated with CQ for 4 h, or infected with SARS-CoV-2 (MOI of 0.05) for 24 h. Scale bar, 2 µm. d Huh7.0 cells were transfected with vector or S-HA, HA-M, HA-N, or HA-E plasmid. Lysates were analysed by immunoblotting. e Interaction between Flag-M and GFP-LC3 in HEK293T cells. f Huh7.0 cells were transfected with the indicated plasmids and infected with SARS-CoV-2 and analysed for the co-localization of BID-GFP and RFP-LC3. g Huh7.0 cells were transfected with the indicated plasmids and analysed for the co-localization of BID-GFP and RFP-LC3. h Huh7.0 cells were transfected with Flag-M plasmid, and mitochondrial fractions were isolated via ultracentrifugation. Cytoplasm (Cyto) and mitochondria (Mito) were analysed by immunoblotting. i HEK293T cells were transfected with the indicated plasmids and infected with Sendai virus for 8 h before the reporter assay was conducted. j HEK293T cells were transfected with the indicated plasmids, and a reporter assay was conducted after transfection. k HEK293T cells were transfected with the indicated plasmids, and a reporter assay was conducted after transfection. Three independent experiments with three technical repetitions were performed. Data are expressed as mean ± SEM (error bars). Statistical analyses used Student’s t test. P < 0.05 was considered statistically significant
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Zhao Z, et al. The interplay between emerging human coronavirus infections and autophagy. Emerg. Microbes Infect. 2021;10:196–205. doi: 10.1080/22221751.2021.1872353. - DOI - PMC - PubMed
    1. Johansen T, Lamark T. Selective autophagy mediated by autophagic adapter proteins. Autophagy. 2011;7:279–296. doi: 10.4161/auto.7.3.14487. - DOI - PMC - PubMed
    1. Wang M, et al. Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro. Cell Res. 2020;30:269–271. doi: 10.1038/s41422-020-0282-0. - DOI - PMC - PubMed
    1. Vomero M, et al. Autophagy modulation in lymphocytes from COVID-19 patients: new therapeutic target in SARS-COV-2 infection. Front Pharmacol. 2020;11:569849. doi: 10.3389/fphar.2020.569849. - DOI - PMC - PubMed
    1. Mohamud Y, et al. The papain-like protease of coronaviruses cleaves ULK1 to disrupt host autophagy. Biochem. Biophys. Res. Commun. 2021;540:75–82. doi: 10.1016/j.bbrc.2020.12.091. - DOI - PMC - PubMed

Publication types