Impact of tea leaves types on antioxidant properties and bioaccessibility of kombucha

Abstract

Five different tea varieties (white, green, oolong, black and pu-erh) were infused, drained and used for kombucha production. Antioxidant capacity, total phenolic content and bioaccessibility values were determined. Extractable, hydrolysable and bioaccessible fractions were used for antioxidant capacity and total phenolic content (TPC) and expressed as µmole TEAC/mL and mg GAE/100 mL, respectively. Antioxidant capacity and TPC were determined by Folin Ciocalteu's, ABTS and CUPRAC methods. In vitro digestion enzymatic extraction was used to determine bioaccessible fractions. It has found that kombucha fermentation with the tea extracts caused to elevate antioxidant capacity, TPC, and bioaccessibility. The green tea kombucha had the highest antioxidant capacity (4153.23 μmole TEAC/mL) and bioaccessibility (50.18%). Therefore, it can be suggested as a good supplement with high nutritional value in kombucha production. In general, TPC content increased significantly. The extractable fractions and bioaccessibility values of the kombucha tea varieties increased due to the fermentation, while the hydrolysable fractions decreased. Antioxidant capacity in the extractable fractions of the green tea kombucha samples was measured to increase by ABTS and CUPRAC methods. Bioaccessibility values of pu-erh tea by ABTS and white tea by CUPRAC were found to increase by the kombucha production.

Keywords: Antioxidant capacity; Bioaccessibility; Fermented beverage; Kombucha; Total phenol.

Fig. 1

Extractable fraction and hydrolysable fraction of TPC of kombucha samples during fermentation (WTK: white tea kombucha; GTK: green tea kombucha; OTK: oolong tea kombucha; BTK: black tea kombucha; PTK: pu-erh tea kombucha)

Fig. 2

Extractable fraction and hydrolysable fraction of antioxidant capacity of kombucha samples during fermentation (μmole TEAC/mL; WTK: white tea kombucha; GTK: green tea kombucha; OTK: oolong tea kombucha; BTK: black tea kombucha; PTK: Pu-erh tea kombucha)

Fig. 3

Bioaccessibility (%) of TPC and antioxidant capacity of kombucha samples during fermentation (WTK: white tea kombucha; GTK: green tea kombucha; OTK: oolong tea kombucha; BTK: black tea kombucha; PTK: pu-erh tea kombucha)