Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Sep-Dec;24(3):582.
doi: 10.4103/jomfp.JOMFP_106_20. Epub 2021 Jan 9.

Expression of p53 at invasive front of oral squamous cell carcinoma and negative histopathological surgical margins to establish correlation at 3-year survival

Madhuri Gawande  1 , Minal Chaudhary  1 , Preethi Sharma  1 , Alka Hande  1 , Swati Patil  1 , Archana Sonone  1
Affiliations

Expression of p53 at invasive front of oral squamous cell carcinoma and negative histopathological surgical margins to establish correlation at 3-year survival

Madhuri Gawande et al. J Oral Maxillofac Pathol. 2020 Sep-Dec.

Abstract

Background: Oral squamous cell carcinoma (OSCC) is the most common malignancy of the oral cavity. The histologic features of OSCC differ from area to area within same tumor, and most prognostic information can be revealed from the invasive front of tumor. The most accepted line of treatment is radical neck dissection. The boundaries of a resected specimen are the surgical margins (SMs), which are excised by the surgeon. The survival outcome is based on the status of these resected SMs. To avoid local recurrence and improve overall survival, it is necessary to attain negative SM. Apart from routine histopathology, the molecular assessment of resected margins has recently gained value which has a promising role for margin surveillance. The value of the use of molecular markers in the routine examination of resection specimens of OSCC has not yet established. It is crucial to identify the percentage of altered cells in SMs which go undetectable in the routine histopathology. It is essential to assess their role in recurrence and survival.

Materials and methods: The study was divided into two groups, i.e., Group I (control group): ten cases of normal oral mucosa and Group II consisted of thirty cases, in which biopsy samples of invasive tumor front and histopathologically negative SM of OSCC were included. Both the groups were subjected to p53 immunohistochemical staining.

Results: There was overexpression of p53 at the deep tumor invasive front of OSCC associated with different histologic grades of malignancy.

Conclusion: The overexpression of p53 at the invading tumor front with clear SMs is associated with poor survival. p53 expression at the tumor front can be a prognostic marker for OSCC.

Keywords: Oral squamous cell carcinoma; p53; survival; tumor-free margins.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Mild staining (×10)
Figure 2
Figure 2
Moderate staining (×10)
Figure 3
Figure 3
Strong staining (×10)
Figure 4
Figure 4
Correlation between immunohistological staining of p53 protein and invasive front grading score
Figure 5
Figure 5
Correlation between immunohistological staining of p53 protein and surgical margins of oral squamous cell carcinoma
Figure 6
Figure 6
Survival of oral squamous cell carcinoma patients at different histological grades
See this image and copyright information in PMC

References

    1. Jiang X, Wu J, Wang J, Huang R. Tobacco and oral squamous cell carcinoma: A review of carcinogenic pathways. Tob Induc Dis. 2019;17:29. - PMC - PubMed
    1. Jadhav KB, Gupta N. Clinicopathological prognostic implicators of oral squamous cell carcinoma: Need to understand and revise. N Am J Med Sci. 2013;5:671–9. - PMC - PubMed
    1. Kumar V, Abbas AK, Fausto N. Neoplasia (Chapter 6) In: Kumar V, Abbas AK, Fausto N, editors. Robbins and Cotran Pathologic basis of Diseases. 7th ed. Philadelphia PA: Saunders; 2004. pp. 302–3.
    1. Muller PA, Vousden KH. Mutant p53 in cancer: New functions and therapeutic opportunities. Cancer Cell. 2014;25:304–17. - PMC - PubMed
    1. Cutilli T, Leocata P, Dolo V, Altobelli E. Evaluation of p53 protein as a prognostic factor for oral cancer surgery. Br J Oral Maxillofac Surg. 2013;51:922–7. - PubMed