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. 2021 Aug:58:101742.
doi: 10.1016/j.mcp.2021.101742. Epub 2021 May 8.

Sensitive on-site detection of SARS-CoV-2 by ID NOW COVID-19

Eva Krause  1 , Andreas Puyskens  1 , Daniel Bourquain  1 , Annika Brinkmann  1 , Barbara Biere  2 , Lars Schaade  1 , Janine Michel  1 , Andreas Nitsche  3
Affiliations

Sensitive on-site detection of SARS-CoV-2 by ID NOW COVID-19

Eva Krause et al. Mol Cell Probes. 2021 Aug.

Abstract

Point of care detection of SARS-CoV-2 is one pillar in a containment strategy and important to break infection chains. Here we report the sensitive, specific and robust detection of SARS-CoV-2 and respective variants of concern by the ID NOW COVID-19 device.

Keywords: COVID-19; Diagnostics; On-site detection; SARS CoV-2.

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Conflict of interest statement

The authors declare that they have no known competing interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Analytical sensitivity of the ID NOW COVID-19 system as determined by binary logistic regression. Positivity rate as well as the 95% CI are plotted vs the CT value for each specimen (blue circles). Red stars indicate specimens from which SARS-CoV-2 could be grown in cell culture, and the red line shows the virus load of 106 genome copies per mL of specimen actually accepted as necessary limit for infectious specimens. The green line indicates the 50% detection probability.
Fig. 2
Fig. 2
Detectability of SARS-CoV-2 Variants of Concern with the ID NOW COVID-19 system. All analyzed VOC can be detected up to CT values that represent genome loads below 800 per mL.
See this image and copyright information in PMC

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