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. 2021 Jan-Feb;42(1):39-43.

Dimethylacetamide in Rooster Semen Cryopreservation

N L S Gatti  1 , C D Corcini  2 , J S Filho  3 , S L Soares  4 , A N Anciuti  3 , R M Barbosa  3 , N W Knabah  3 , A T Tavares  5 , D C Bongalhardo  6 , A S Varela Junior  6
Affiliations
  • PMID: 33973991

Dimethylacetamide in Rooster Semen Cryopreservation

N L S Gatti et al. Cryo Letters. 2021 Jan-Feb.

Abstract

Background: Sperm cryopreservation of cockerels is a major challenge, and so far there is no adequate information to enable commercial use of frozen semen.

Objective: To test the toxicity of dimethylacetamide (DMA).

Materials and methods: DMA was added at 3%, 6%, 9% and 12% to the freezing diluent, and maintained for equilibration with the semen sample for 1 min, 3 min, 5 min, 7 min and 9 min prior to freezing. Thawed semen was evaluated for kinetic characteristics by computer-assisted semen analysis (CASA) and for structural and functional properties by flow cytometry (plasma membrane rupture, mitochondrial functionality and plasma membrane functionality).

Results and conclusion: The addition of 6% DMA for 3-min equilibration resulted in the highest total and progressive motility, 42.0% and 36.9%, respectively. The point of intersection between a good protection and low plasma membrane rupture was obtained with the addition of 6% of DMA for 3-min equilibration with the rooster semen.

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