Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Nov;40(11):2447-2453.
doi: 10.1007/s10096-021-04267-6. Epub 2021 May 11.

Rapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow-based detection

Abdulrahman K S Ayfan  1   2 , Joanne Macdonald  3 , Patrick N A Harris  1   4 , Claire Heney  4 , David L Paterson  1 , Ella Trembizki  1 , Claire Y T Wang  5 , David M Whiley  1   4 , Hosam M Zowawi #  1   6   7 , Adam D Irwin #  8   9
Affiliations

Rapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow-based detection

Abdulrahman K S Ayfan et al. Eur J Clin Microbiol Infect Dis. 2021 Nov.

Abstract

Carbapenemase-producing organisms (CPOs) pose a serious clinical threat and rapid detection tools are essential to aid in patient management. We developed rapid and simple molecular tests to detect blaNDM-type and blaVIM-type carbapenemase genes using recombinase polymerase amplification (RPA) combined with a lateral flow detection. The tests could provide results in approximately 15 min when using DNA extracts, with limits of detection of 9.2 copies/μl for the blaNDM-type assay and 7.5 copies/μl for blaVIM-type assay, and successfully detected all isolates harbouring the carbapenemase encoding genes in a panel of 57 isolates. These RPA tests may be suitable for use in low-resource settings to tailor rapid implementation of infection control precautions and antibiotic stewardship.

Keywords: Carbapenemase; Lateral flow; NDM; Point-of-care; Recombinase polymerase amplification; VIM.

PubMed Disclaimer

References

    1. CDC. Biggest Threats and Data. 2019 26-04-2021]; Available from: https://www.cdc.gov/drugresistance/biggest-threats.html .
    1. WHO. WHO publishes list of bacteria for which new antibiotics are urgently needed. 2017 26-04-2021]; Available from: https://www.who.int/news/item/27-02-2017-who-publishes-list-of-bacteria-... .
    1. CDC. Clinicians: Information about CRE. 2019 26-04-2021]; Available from: https://www.cdc.gov/hai/organisms/cre/cre-clinicians.html .
    1. WHO. Guidelines for the prevention and control of carbapenem-resistant Enterobacteriaceae, Acinetobacter baumannii and Pseudomonas aeruginosa in health care facilities. 2017 26-04-2021]; Available from: https://www.who.int/infection-prevention/publications/guidelines-cre/en/ .
    1. Banerjee R, Humphries R (2017) Clinical and laboratory considerations for the rapid detection of carbapenem-resistant Enterobacteriaceae. Virulence 8(4):427–439 - DOI

Publication types

MeSH terms

LinkOut - more resources