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. 2021:2296:333-350.
doi: 10.1007/978-1-0716-1358-0_19.

Production, Detection, Extraction, and Quantification of Polyene Antibiotics

Eva G Barreales  1 , Jesús F Aparicio  2
Affiliations

Production, Detection, Extraction, and Quantification of Polyene Antibiotics

Eva G Barreales et al. Methods Mol Biol. 2021.

Abstract

Polyene antibiotics are macrolide antifungal compounds obtained by fermentation of producer Streptomyces strains. Here we describe commonly used methods for polyene production, detection, and their subsequent extraction and purification. While bioassays are used to detect these compounds based on their biological activity, quantification by spectrophotometry or high-performance liquid chromatography (HPLC ) relies on their physiochemical properties and is more reliable.

Keywords: Antifungal agent; Bioassay; HPLC; Polyene macrolide; Streptomyces; UV-visible spectrum.

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