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. 2021 May 5:14:1775-1787.
doi: 10.2147/JIR.S298244. eCollection 2021.

Investigation of Wound Healing and Anti-Inflammatory Activities of Solvent Fractions of 80% Methanol Leaf Extract of Achyranthes aspera L. (Amaranthaceae) in Rats

Teklie Mengie  1 , Solomon Mequanente  2 , Dereje Nigussie  3 , Belete Legesse  4 , Eyasu Makonnen  2   4
Affiliations

Investigation of Wound Healing and Anti-Inflammatory Activities of Solvent Fractions of 80% Methanol Leaf Extract of Achyranthes aspera L. (Amaranthaceae) in Rats

Teklie Mengie et al. J Inflamm Res. .

Retraction in

Abstract

Introduction: The various fractions of leaves of Achyranthes aspera L. (A. aspera) have not yet been explored scientifically for in-vivo wound healing and anti-inflammatory activities. The objective of this study was, therefore, to evaluate in-vivo wound healing and anti-inflammatory activities of solvent fractions of 80% methanol leaf extract of A. aspera in rats.

Methods: The 80% methanol leaf extract of A. aspera was fractionated with chloroform, n-butanol and water. Wound healing and anti-inflammatory activities were evaluated using excision and incision wound models, rat paw edema and cotton pellet-induced granuloma models, respectively. For wound healing activity, fractions were evaluated at 5 and 10% ointments. The positive control groups were treated with nitrofurazone 0.2% ointment. Simple ointment treated for excision wound model and untreated for incision wound model rats were assigned as negative controls. For anti-inflammatory activity, fractions were evaluated at 100, 200 and 400mg/kg. Positive control groups were treated with indomethacin 10mg/kg for both rat paw edema and cotton pellet-induced granuloma models. The 2% Tween 80 treated rats were assigned as negative controls for both anti-inflammatory activity models. All groups comprised of 6 rats and treatment administrations were made topically and orally for evaluation of wound healing and anti-inflammatory activities.

Results: The 10% w/w chloroform fraction ointment revealed a high percentage of wound contraction and reduced period of epithelialization (p <0.01). Chloroform fraction was also found to be the most active fraction, which demonstrated the maximum percentage inhibition of edema (52.50%; p <0.01) and transudative and proliferative component of chronic inflammation (37.52 and 52.81%; p <0.01), which was comparable to indomethacin.

Conclusion: Data obtained from this study collectively indicated that a chloroform fraction of 80% methanol leaf extract of A. aspera possessed significant wound healing and anti-inflammatory activities.

Keywords: Achyranthes aspera; carrageenan-induced paw edema; cotton pellet granuloma; wound healing.

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Conflict of interest statement

The authors reported no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Achyranthes aspera from site of collection (Captured on 20/04/2019).
Figure 2
Figure 2
Excision wound on rats treated with solvent fraction ointments of leaves of A. aspera in rats.
See this image and copyright information in PMC

References

    1. Farahpour MR, Habibi M. Evaluation of the wound healing activity of an ethanolic extract of Ceylon cinnamon in mice. Vet Med (Praha). 2012;57(1):53–57. doi:10.17221/4972-VETMED - DOI
    1. Bowler PG, Davies BJ. The microbiology of infected and noninfected leg ulcers. Int J Dermatol. 1999;38(8):573–578. doi:10.1046/j.1365-4362.1999.00738.x - DOI - PubMed
    1. Bowler PG. Wound pathophysiology, infection and therapeutic options. Ann Med. 2002;34(6):419–427. doi:10.1080/078538902321012360 - DOI - PubMed
    1. Lazarus GS, Cooper DM, Knighton DR, et al. Definitions and Guidelines for Assessment of Wounds and Evaluation of Healing Back. Arch Dermatol. 1994;130:489–493. doi:10.1001/archderm.1994.01690040093015 - DOI - PubMed
    1. Garg P, Sardana S. Pharmacological and therapeutic effects of ocimum sanctum. Eur J Pharm Med Res. 2016;3(8):637–640.

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