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. 2021 Jun 7;57(45):5594-5596.
doi: 10.1039/d1cc00876e. Epub 2021 May 13.

Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure

Katsuhiko Abe  1 , Hiroshi Sugiyama, Masayuki Endo
Affiliations
Free article

Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure

Katsuhiko Abe et al. Chem Commun (Camb). .
Free article

Abstract

We demonstrated the photo-controlled sequence-selective dsDNA cleavage using a DNA origami structure with Cas9 nuclease. The activity of Cas9 incorporated inside the ring-shaped DNA origami was completely suppressed. After photoirradiation, Cas9 was released, and its activity for selective dsDNA cleavage was restored.

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