High malic enzyme activity in tumor cells and its cross-reaction with anti-pigeon liver malic enzyme serum
- PMID: 3398839
- DOI: 10.1007/BF02424560
High malic enzyme activity in tumor cells and its cross-reaction with anti-pigeon liver malic enzyme serum
Abstract
Rabbit antibodies against pigeon liver malic enzyme (EC 1.1.1.40) were prepared. The antiserum gave single precipitation line with crude pigeon liver extract. Cross reaction was observed with partially purified malic enzyme or crude extract from chicken liver. Positive cross reaction was also observed with the concentrated cytosolic fraction of two human carcinoma cell lines which were demonstrated to contain high malic enzyme activity. All other proteins examined did not react with the antibodies. When purified pigeon liver malic enzyme was mixed with the antiserum in vitro, a time-dependent inactivation of the enzyme activity was observed. Protection of the enzyme activity against antiserum inactivation was afforded by NADP+ or L-malate. Metal Mn2+ gave little protection.
Similar articles
-
Cloning and expression of pigeon liver cytosolic NADP(+)-dependent malic enzyme cDNA and some of its abortive mutants.Arch Biochem Biophys. 1994 Apr;310(1):158-66. doi: 10.1006/abbi.1994.1152. Arch Biochem Biophys. 1994. PMID: 8161199
-
Equilibrium substrate binding studies of the malic enzyme of pigeon liver. Equivalence of nucleotide sites and anticooperativity associated with the binding of L-malate to the enzyme-manganese(II)-reduced nicotinamide adenine dinucleotide phosphate ternary complex.Biochemistry. 1980 Mar 4;19(5):951-62. doi: 10.1021/bi00546a020. Biochemistry. 1980. PMID: 7356971
-
Affinity cleavage at the putative metal-binding site of pigeon liver malic enzyme by the Fe(2+)-ascorbate system.Biochemistry. 1994 Jun 28;33(25):7931-6. doi: 10.1021/bi00191a021. Biochemistry. 1994. PMID: 8011656
-
Periodate-oxidized 3-aminopyridine adenine dinucleotide phosphate as a fluorescent affinity label for pigeon liver malic enzyme.J Biol Chem. 1989 Jan 5;264(1):280-7. J Biol Chem. 1989. PMID: 2909520
-
Hormonal regulation of the activity of the fatty acid synthesizing system and of the malic enzyme concentration in liver cells-1,2.Fed Proc. 1975 Feb;34(2):117-23. Fed Proc. 1975. PMID: 803905 Review. No abstract available.
Cited by
-
Repressing malic enzyme 1 redirects glucose metabolism, unbalances the redox state, and attenuates migratory and invasive abilities in nasopharyngeal carcinoma cell lines.Chin J Cancer. 2012 Nov;31(11):519-31. doi: 10.5732/cjc.012.10088. Epub 2012 Nov 1. Chin J Cancer. 2012. PMID: 23114090 Free PMC article.
-
Nonidentity of the cDNA sequence of human breast cancer cell malic enzyme to that from the normal human cell.J Protein Chem. 1996 Apr;15(3):273-9. doi: 10.1007/BF01887116. J Protein Chem. 1996. PMID: 8804575
-
Biophysical characterization of the dimer and tetramer interface interactions of the human cytosolic malic enzyme.PLoS One. 2012;7(12):e50143. doi: 10.1371/journal.pone.0050143. Epub 2012 Dec 21. PLoS One. 2012. PMID: 23284632 Free PMC article.
-
Proposed cycles for functional glutamate trafficking in synaptic neurotransmission.Neurochem Int. 2008 Mar-Apr;52(4-5):809-25. doi: 10.1016/j.neuint.2007.09.015. Epub 2007 Oct 2. Neurochem Int. 2008. PMID: 18006192 Free PMC article.