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. 2021 Jul;19(7):1294-1296.
doi: 10.1111/pbi.13629. Epub 2021 Jun 4.

The Arabidopsis immune receptor EFR increases resistance to the bacterial pathogens Xanthomonas and Xylella in transgenic sweet orange

Letícia Kuster Mitre  1   2 , Natália Sousa Teixeira-Silva  1 , Katarzyna Rybak  3 , Diogo Maciel Magalhães  1   2 , Reinaldo Rodrigues de Souza-Neto  1   2 , Silke Robatzek  3 , Cyril Zipfel  4   5 , Alessandra Alves de Souza  1
Affiliations

The Arabidopsis immune receptor EFR increases resistance to the bacterial pathogens Xanthomonas and Xylella in transgenic sweet orange

Letícia Kuster Mitre et al. Plant Biotechnol J. 2021 Jul.
No abstract available

Keywords: broad-spectrum disease resistance; citrus canker; citrus variegated chlorosis; outer membrane vesicle; pathogen-associated molecular pattern; pattern recognition receptor.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Arabidopsis EFR responds to Xcc and Xfp, inducing broad‐spectrum resistance in transgenic sweet orange. (a) A. thaliana efr‐1 is strongly impaired in Xff‐induced ROS burst (n = 10). (b) Xff OMVs repress seedling growth in Arabidopsis wild‐type Col‐0 but not efr‐1 and bak1‐5. (c) Xff bacterial titres are higher in efr‐1 measured as relative expression of the Xff HL5/HL6 locus 14 days after petiole infection. (d,e) ROS production in Arabidopsis Col‐0 (d) and N.tabacum expressing EFR (e) triggered by elf peptides (elf18Ec, elf18Xcc and elf26Xfp). (f) Transgenic integration confirmed by histochemical gus assay and relative expression of EFR measured by RT‐qPCR normalized by the expression of cyclophilin in Valencia transgenic citrus lines. (g) Alignment of the EF‐Tu‐derived elf18 sequences from E. coli, X citri and X.fastidiosa compared to the minimal peptide (in red, where X is any amino acid) required for full EFR activation. Amino acids in blue represent substitutions. (h) ROS production of transgenic citrus lines in response to elf peptides (elf18Ec, elf18Xcc and elf26Xfp). (i) MAPK activation and (j) defence gene induction in citrus‐EFR lines after elf treatment. (k) CC symptom development and (l) bacterial growth in detached leaves of citrus‐EFR lines infected with Xcc. Circles represent details in bright‐field (upper circle) and under GFP‐fluorescence (lower circle). (m) CVC symptomatology and (n) bacterial population disease severity score 18 months after Xfp inoculation. RLU: relative light units. All the experiments were performed three times with at least n = 3 with similar results.
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