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. 1988 Jul 25;16(14A):6607-16.
doi: 10.1093/nar/16.14.6607.

The helical repeat of underwound DNA in solution

S M Richardson  1 , T C Boles, N R Cozzarelli
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Free PMC article

The helical repeat of underwound DNA in solution

S M Richardson et al. Nucleic Acids Res. .
Free PMC article

Abstract

Closed circular DNA was relaxed with a topoisomerase in the presence of varying concentrations of the intercalating dye, ethidium bromide, to create underwound, planar DNA rings. We directly determined the helical repeat of these DNA molecules by the Gaussian center method and found that it varied as a simple predicted function of the degree of underwinding and the helical repeat of relaxed, dye-free DNA. We discuss these results in light of a recent mathematical treatment of DNA structure which predicts that the helical repeat of supercoiled DNA molecules in solution obeys the same function.

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