Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Fall;11(Suppl 1):S101-S106.
doi: 10.34172/jlms.2020.S16. Epub 2020 Dec 30.

An Investigation of Post-radiation Gene Expression Profiles: A System Biology Study

Reza Vafaee  1 , Abdolrahim Nikzamir  2 , Mohhamadreza Razzaghi  3 , Sina Rezaei Tavirani  4 , Alireza Ahmadzadeh  2 , MohammadAli Emamhadi  5
Affiliations

An Investigation of Post-radiation Gene Expression Profiles: A System Biology Study

Reza Vafaee et al. J Lasers Med Sci. 2020 Fall.

Abstract

Introduction: Genomics and bioinformatics are useful methods for exploring unclear aspects of radiation effects on biological systems. Many radiation-induced alterations in irradiated samples are post-radiation time-dependent. This study aims to evaluate the post-irradiation effects of the gamma ray on human Jurkat cells. Methods: Gene expression profiles of the samples harvested 6 and 24 hours after radiation to find the critical differential expressed genes and the related pathways. Samples are provided from Gene Expression Omnibus (GEO) and analyzed by ClueGO. Results: Twnety-nine critical genes were determined as the important affected genes and 7 classes of related pathways were introduced. CCNE2, PSMD11, CDC25C, ANAPC1, PLK1, AURKA, and CCNB1 that were associated with more than 6 pathways were related to one of the determined pathway groups. Conclusion: Cell protecting pathways were associated with the genes (HSPA5, HSPA8, HSP90B1, HMMR, CEBPB, RXRA, and PSMD11) which were related to the minimum numbers of pathways. The finding of this study corresponds to repair processes which depend on post-radiation time. It seems these sets of genes are suitable candidates for further investigation.

Keywords: Bioinformatics; Dysregulation; Gene expression; Pathway; Radiation.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Figure 2
Figure 2
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Kumar DS, Chakrabarty D, Verma AK, Banerji BK. Gamma ray induced chromosomal aberrations and enzyme related defense mechanism in Allium cepa L. Caryologia G CitolCitosistematicaCitogenet. 2011;64(4):388–97.
    1. Teoule R. Radiation-induced DNA damage and its repair. Int J Radiat Biol Relat Stud Phys Chem Med. 1987;51(4):573–89. doi: 10.1080/09553008414552111. - DOI - PubMed
    1. Kadhim MA, Moore SR, Goodwin EH. Interrelationships amongst radiation-induced genomic instability, bystander effects, and the adaptive response. Mutat Res. 2004;568(1):21–32. doi: 10.1016/j.mrfmmm.2004.06.043. - DOI - PubMed
    1. Matsui A, Kobayashi J, Kanno SI, Hashiguchi K, Miyaji M, Yoshikawa Y. et al. Oxidation resistance 1 prevents genome instability through maintenance of G2/M arrest in gamma-ray-irradiated cells. J Radiat Res. 2020;61(1):1–13. doi: 10.1093/jrr/rrz080. - DOI - PMC - PubMed
    1. Georgakilas AG, Pavlopoulou A, Louka M, Nikitaki Z, Vorgias CE, Bagos PG. et al. Emerging molecular networks common in ionizing radiation, immune and inflammatory responses by employing bioinformatics approaches. Cancer Lett. 2015;368(2):164–72. doi: 10.1016/j.canlet.2015.03.021. - DOI - PubMed

LinkOut - more resources