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. 2021 Apr 29:11:654852.
doi: 10.3389/fcimb.2021.654852. eCollection 2021.

Co-Occurrence of NDM-5 and RmtB in a Clinical Isolate of Escherichia coli Belonging to CC354 in Latin America

Agustina Costa  1 , Roque Figueroa-Espinosa  1   2 , Florencia Gaudenzi  3 , Nilton Lincopan  4 , Bruna Fuga  4 , Barbara Ghiglione  1   2 , Gabriel Gutkind  1   2 , José Di Conza  1   2
Affiliations

Co-Occurrence of NDM-5 and RmtB in a Clinical Isolate of Escherichia coli Belonging to CC354 in Latin America

Agustina Costa et al. Front Cell Infect Microbiol. .

Abstract

New Delhi metallo-β-lactamase (NDM)-producing isolates are usually resistant to most β-lactams and other antibiotics as a result of the coexistence of several resistance markers, and they cause a variety of infections associated to high mortality rates. Although NDM-1 is the most prevalent one, other variants are increasing their frequency worldwide. In this study we describe the first clinical isolate of NDM-5- and RmtB-producing Escherichia coli in Latin America. E. coli (Ec265) was recovered from a urine sample of a female outpatient. Phenotypical and genotypical characterization of resistance markers and conjugation assays were performed. Genetic analysis of Ec265 was achieved by whole genome sequencing. Ec265 belonging to ST9693 (CC354), displayed resistance to most β-lactams (including carbapenems), aminoglycosides (gentamicin and amikacin), and quinolones. Several resistance genes were found, including blaNDM-5 and rmtB, located on a conjugative plasmid. blaNDM-5 genetic context is similar to others found around the world. Co-transfer of multiple antimicrobial resistance genes represents a particular challenge for treatment in clinical settings, whereas the spread of pathogens resistant to last resort antibiotics should raise an alarm in the healthcare system worldwide.

Keywords: Escherichia coli; NDM-5; RmtB; antibiotic multi-resistance; metallo-β-lactamase.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Schematic representation of bla NDM contexts of Ec265 and other E. coli human isolates worldwide disseminated: (A) GenBank accession no. JQ364967 (skin, France); (B) Ec265 strain isolated from urine, in Argentina (GenBank accession no. JACXXI000000000; (C) the same sequence correspond to three clinical strains isolated from rectal swab in Canada (GenBank accession no. CP023871), blood in Myanmar (GenBank accession no. AP018147), and urine in Italy (GenBank accession no. MN007141). The light-blue bands indicate identical gene arrangement.
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References

    1. Aung M. S., San N., Maw W. W., San T., Urushibara N., Kawaguchiya M., et al. . (2018). Prevalence of Extended-Spectrum Beta-Lactamase and Carbapenemase Genes in Clinical Isolates of Escherichia Coli in Myanmar: Dominance of bla NDM-5 and Emergence of Bla oxa-181 . Microbial Drug Resist. 19, 284–293. 10.1089/mdr.2017.0387 - DOI - PubMed
    1. Basu S. (2020). Variants of the New Delhi Metallo-β-Lactamase: New Kids on the Block. Future Microbiol. 15 (7), 465–467. 10.2217/fmb-2020-0035 - DOI - PubMed
    1. Berçot B., Poirel L., Nordmann P. (2011). Updated Multiplex Polymerase Chain Reaction for Detection of 16S rRNA Methylases: High Prevalence Among NDM-1 Producers. Diagn. Microbiol. Infect. Dis. 71 (4), 442–425. 10.1016/j.diagmicrobio.2011.08.016 - DOI - PubMed
    1. Bonnin R. A., Poirel L., Carattoli A., Nordmann P. (2012). Characterization of an IncFII Plasmid Encoding NDM-1 From Escherichia Coli ST131. PLoS One. 7 (4), e34752. 10.1371/journal.pone.0034752 - DOI - PMC - PubMed
    1. Chavda K. D., Chen L., Jacobs M. R., Bonomo R. A., Kreiswirth B. R. (2016). Molecular Diversity and Plasmid Analysis of KPC-producing Escherichia Coli . Antimicrob. Agents Chemother. 60 (7), 4073–4081. 10.1128/AAC.00452-16 - DOI - PMC - PubMed

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