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. 2021 Apr 30:9:661321.
doi: 10.3389/fped.2021.661321. eCollection 2021.

Suitability of Placental Blood Samples of Newborns for Pre-Transfusion Testing

Rana Alissa  1 , Patty D Williams  1 , Erika L Baker  1 , Jennifer A Hipp  1 , Jinous Saremian  2 , Agnes E Aysola  2
Affiliations

Suitability of Placental Blood Samples of Newborns for Pre-Transfusion Testing

Rana Alissa et al. Front Pediatr. .

Abstract

Objective: To show concordance between heel stick and placental blood sample pairs for newborns' pre-transfusion testing and to validate placental blood's tube and gel methodology. Methods: Placental samples were collected for pre-transfusion testing at birth from 78 singleton and twin newborns admitted to our Mother-Baby Unit to compare with the results of heel stick samples taken from same newborns. Gestational age ≥35 weeks, weight ≥2,000 g. The study was approved by the Institutional Review Board (IRB). Informed consent was obtained from newborn parents. ABO blood group, Rhesus factor (Rh), direct antiglobulin test (DAT), and antibody screen were performed. Ortho ProVue Analyzer was used for tube and gel methods. McNemar's test for paired categorical data was performed. Results: One hundred percent concordance in 78 pairs for ABO and Rh. Seventy-four pairs were tested for antibodies, 72 were both negative, 1 was both positive, and 1 gave discordant result. Ninety-nine percent concordance, p = 0.999. Sixty-five pairs were both DAT negative, seven were both DAT positive, and six gave discordant results. Ninety-two percent concordance, p = 0.68. Placental blood gave identical results comparing tube with gel methods. Conclusions: Placental blood is suitable for pre-transfusion testing and can replace heel sticks. Placental blood tube and gel methods are validated.

Keywords: heel stick; less invasive; natural; placental blood; pre-transfusion testing.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Collecting blood sample from placenta.
See this image and copyright information in PMC

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