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. 2021 May 3:2021:5553486.
doi: 10.1155/2021/5553486. eCollection 2021.

Experimental Study on the Correlation between miRNA-373 and HIF-1 α, MMP-9, and VEGF in the Development of HIE

Chun-Yang Liu  1   2 , Hisham Al-Ward  1 , Francine Ngaffo Mekontso  3 , Ning Liu  1 , Hui-Qin Zeng  4 , Min Liu  5 , Zi-Rui Yu  6 , Lin Zhang  7 , Yu-Chun Han  7 , Hui Xu  1
Affiliations

Experimental Study on the Correlation between miRNA-373 and HIF-1 α, MMP-9, and VEGF in the Development of HIE

Chun-Yang Liu et al. Biomed Res Int. .

Retraction in

Abstract

Introduction: Microribonucleic acids (miRNAs) have short (approximately 18 to 25) nucleotides and are evolutionarily conserved and endogenously expressed RNAs belonging to a family of noncoding RNA molecules. miRNA-373 regulates cell proliferation, migration, apoptosis, invasion, and repairing damaged DNA after hypoxia stress. Neonatal hypoxic-ischemic encephalopathy (HIE) refers to perinatal asphyxia caused by partial or complete hypoxia, reduced or suspended cerebral blood flow, and fetal or neonatal brain damage. We aim to investigate the relationship between miRNA-373 and HIF-1α, between miRNA-373 MMP-9, and between miRNA-373 VEGF in the occurrence and development of HIE.

Methods: Human (children) samples were divided into four groups (n = 15 in each group) according to HIE severity. The patient group was divided into middle, moderate, and severe HIE groups. The control group included healthy children or children with nonneurological diseases. The expressions of miRNA-373, HIF-1α, MMP-9, and VEGF were assayed in the serum samples.

Results: Our study showed a strong relationship between miRNA-373 and HIF-1α, between miRNA-373 and MMP-9, and between miRNA-373 and VEGF. The expression levels of miRNA-373, HIF-1α, MMP-9, and VEGF in the HIE groups were much higher than those of the control group.

Conclusion: The increased change in miRNA-373 expression has a certain diagnostic significance on neonatal HIE. In the occurrence and development of HIE, miRNA-373 is positively correlated with HIF-1α, MMP-9, and VEGF.

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Conflict of interest statement

The authors have no conflict of interest to declare.

Figures

Figure 1
Figure 1
cDNA agarose gel electrophoresis image. M: marker; 1 and 2: control group; 3–7: severe group; 8–12: moderate group; 13–17: mild group.
Figure 2
Figure 2
(a) Amplification curve. (b) Melting curve of miR-373.
Figure 3
Figure 3
Fluorescence quantitative PCR to detect the expression of miR-373 in the sample. ∗The difference is statistically significant compared with the control group (P < 0.05).
Figure 4
Figure 4
Comparison of serum HIF-1α levels in each group (nmol/mL). ∗∗The difference is statistically significant compared with the control group (P < 0.01).
Figure 5
Figure 5
Comparison of MMP-9 levels in serum of each group (nmol/mL). ∗∗The difference is statistically significant compared with the control group (P < 0.01).
Figure 6
Figure 6
Comparison of serum VEGF levels in each group (nmol/mL). ∗∗The difference is statistically significant compared with the control group (P < 0.01).
Figure 7
Figure 7
Correlation analysis of patient (human) serum miRNA-373 and HIF-1α, MMP-9, and VEGF. L: mild group; M: moderate group; S: severe group.
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