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. 2021 Apr 21;2(2):100476.
doi: 10.1016/j.xpro.2021.100476. eCollection 2021 Jun 18.

Quantifying interfacial substrate interactions via surface energy analyses

T Brian Cavitt  1 , Jasmine G Carlisle  1 , Rachel A Brooks  1 , Lauren G Scott  1 , Pooja R Patel  1
Affiliations

Quantifying interfacial substrate interactions via surface energy analyses

T Brian Cavitt et al. STAR Protoc. .

Abstract

Determination of a substrate's surface energy profile is a facile and inexpensive method to indicate the substrate's interfacial thermodynamics with another substance (e.g., microorganisms, biomacromolecules, medical devices, etc). The following protocol details a goniometric method to calculate a substrate's surface energy profile which (1) directly correlates to a substrate's interfacial Gibbs energy (ΔG) and (2) predicts the interfacial interactions with other substances. We also provide a calculation template using advanced mathematics to expedite surface energy profile determination. For complete details on the use and execution of this protocol, please refer to Cavitt et al. (2020).

Keywords: Biophysics; Energy; Material sciences; Microbiology; Physics.

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Conflict of interest statement

The authors declare no competing interests.

Figures

None
Graphical abstract
Figure 1
Figure 1
Setup for coating glass slides with OTS solution for adhering a biomacromolecule to a nonpolar substrate
Figure 2
Figure 2
Contact angle measurement station Figure 2 labels the components used to obtain the contact angle measurements where (A) is the mounted portable electronic device, (B) is the macrolens clip, (C) is the sample platform, (D) adjustable LED lamp, (E) Bunsen burner to remove humidity when lit, and (F) climate- controlled sample conditioning chamber.
Figure 3
Figure 3
Example contact angles and measurement via a screenshot of the Photo Protractor app Note: The baseline of the angles often needs to be slightly adjusted to ensure planarity with substrate (i.e., contact angle adjusts to 54.284°).
Figure 4
Figure 4
Depiction of Sheet 1 "INPUT Highlighted ID & CAs” illustrating the highlighted regions requiring the input of the sample name and contact angles, CAs (See also Data S1)
Figure 5
Figure 5
Depiction of Sheet 2 “Calculations” illustrating the breadth of calculations performed to obtain the surface energy profile boxed at bottom left (See also Data S1)
Figure 6
Figure 6
Depiction of Sheet 3 “Surface Energy Results” providing surface energy data in tabular form for facile reporting (See also Data S1)
See this image and copyright information in PMC

References

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