Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jul;61(7):652-661.
doi: 10.1002/jobm.202100090. Epub 2021 May 16.

Contribution to determination of extracellular DNA origin in the biofilm matrix

Viviana Svarcova  1 , Kamila Zdenkova  1 , Martina Sulakova  1 , Katerina Demnerova  1 , Jarmila Pazlarova  1
Affiliations

Contribution to determination of extracellular DNA origin in the biofilm matrix

Viviana Svarcova et al. J Basic Microbiol. 2021 Jul.

Abstract

This study is focused on the analysis of extracellular DNA (eDNA) from a biofilm matrix formed by Staphylococcus aureus, Listeria monocytogenes, and Salmonella enterica. The presence of eDNA in the biofilm of all the studied strains was confirmed by confocal laser scanning microscopy using fluorescent dyes with high affinity to nucleic acid. The protocol for eDNA isolation from the biofilm matrix was established, and subsequent characterization of the eDNA was performed. The purified eDNA obtained from the biofilm matrix of all three microorganisms was compared to the genomic DNA (gDNA) isolated from relevant planktonic grown cells. The process of eDNA isolation consisted of biofilm cultivation, its collection, sonication, membrane filtration, dialysis, lyophilisation, and extraction of DNA separated from the biofilm matrix with cetyltrimethylammonium bromide. An amplified fragment length polymorphism (AFLP) was used for comparing eDNA and gDNA. AFLP profiles showed a significant similarity between eDNA and gDNA at the strain level. The highest similarity, with a profile concordance rate of 94.7% per strain, was observed for S. aureus, L. monocytogenes, and S. enterica exhibited lower profiles similarity (78% and 60%, respectively). The obtained results support the hypothesis that the eDNA of studied bacterial species has its origin in the gDNA.

Keywords: Listeria monocytogenes; Salmonella enterica; Staphylococcus aureus; AFLP; biofilm; extracellular DNA.

PubMed Disclaimer

References

REFERENCES

    1. Costerton JW , Cheng KJ , Geesey GG , Ladd TI , Nickel JC , Dasgupta M , et al. Bacterial biofilms in nature and disease. Annu Rev Microbiol. 1987;41:435-64.
    1. Flemming HC . The perfect slime. Colloids Surf B Biointerfaces. 2011;86:251-9.
    1. Das T , Sharma PK , Busscher HJ , van der Mei HC , Krom BP . Role of extracellular DNA in initial bacterial adhesion and surface aggregation. Appl Environ Microbiol. 2010;76:3405-8.
    1. Liu H , Yang Y , Li Y , Xie Z , Shen P . Involvement of DNA in biofilm formation: features and mechanism of DNA adsorption to bacterial surfaces. J Biosci Med. 2011;1:1-10.
    1. da Silva EP , de Martinis EC . Current knowledge and perspectives on biofilm formation: the case of Listeria monocytogenes . Appl Microbiol Biotechnol. 2013;97:957-68.

MeSH terms

LinkOut - more resources