Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar;54(2):427-437.
doi: 10.1111/evj.13450. Epub 2021 May 18.

The effect of ischaemic postconditioning on mucosal integrity and function in equine jejunal ischaemia

Nicole Verhaar  1 , Gerhard Breves  2 , Marion Hewicker-Trautwein  3 , Christiane Pfarrer  4 , Karl Rohn  5 , Marion Burmester  2 , Nadine Schnepel  2 , Stephan Neudeck  1 , Lara Twele  1 , Sabine Kästner  1   6
Affiliations

The effect of ischaemic postconditioning on mucosal integrity and function in equine jejunal ischaemia

Nicole Verhaar et al. Equine Vet J. 2022 Mar.

Abstract

Background: Ischaemic postconditioning (IPoC) has been shown to ameliorate ischaemia reperfusion injury in different species and tissues.

Objectives: To assess the feasibility of IPoC in equine small intestinal ischaemia and to assess its effect on histomorphology, electrophysiology and paracellular permeability.

Study design: Randomised in vivo experiment.

Methods: Experimental jejunal ischaemia was induced for 90 min in horses under general anaesthesia. In the control group (C; n = 7), the jejunum was reperfused without further intervention. In the postconditioning group (IPoC; n = 7), reocclusion was implemented following release of ischaemia by clamping the mesenteric vessels in three cycles of 30 seconds. This was followed by 120 minutes of reperfusion in both groups. Intestinal microperfusion and oxygenation was measured during IPoC using spectrophotometry and Doppler flowmetry. Histomorphology and histomorphometry of the intestinal mucosa were assessed. Furthermore, electrophysiological variables and unidirectional flux rates of 3 H-mannitol were determined in Ussing chambers. Western blot analysis was performed to determine the tight junction protein levels of claudin-1, claudin-2 and occludin in the intestinal mucosa. Comparisons between the groups and time points were performed using a two-way repeated measures analysis of variance (ANOVA) or non-parametric statistical tests for the ordinal and not normally distributed data (significance P < .05).

Results: IPoC significantly reduced intestinal microperfusion during all clamping cycles yet affected oxygen saturation only during the first cycle. After reperfusion, Group IPoC showed significantly less mucosal villus denudation (mean difference 21.5%, P = .02) and decreased mucosal-to-serosal flux rates (mean difference 15.2 nM/cm2 /h, P = .007) compared to Group C. There were no significant differences between the groups for the other tested variables.

Main limitations: Small sample size, long-term effects were not investigated.

Conclusions: Following IPoC, the intestinal mucosa demonstrated significantly less villus denudation and paracellular permeability compared to the untreated control group, possibly indicating a protective effect of IPoC on ischaemia reperfusion injury.

Keywords: colic; conditioning; horse; protective; reperfusion; strangulation.

PubMed Disclaimer

References

REFERENCES

    1. LouB, CuiY, GaoH, ChenM. Meta-analysis of the effects of ischemic postconditioning on structural pathology in ST-segment elevation acute myocardial infarction. Oncotarget. 2018;9:8089.
    1. ZhaoZ-Q, CorveraJS, HalkosME, KerendiF, WangN-P, GuytonRA, et alInhibition of myocardial injury by ischemic postconditioning during reperfusion: comparison with ischemic preconditioning. Am J Physiol Heart Circ Physiol. 2003;285:579-88.
    1. JiaZ, LianW, ShiH, CaoC, HanS, WangK, et alIschemic postconditioning protects against intestinal ischemia/reperfusion injury via the HIF-1alpha/miR-21 Axis. Sci Rep. 2017;7:16190.
    1. ZhaoJJ, XiaoH, ZhaoWB, ZhangXP, XiangY, YeZJ, et alRemote ischemic postconditioning for ischemic stroke: a systematic review and meta-analysis of randomized controlled trials. Chinese Med J. 2018;131(8):956-65.
    1. Nepper-ChristensenL, HøfstenDE, HelqvistS, LassenJF, TilstedH-H, HolmvangL, et alInteraction of ischaemic postconditioning and thrombectomy in patients with ST-elevation myocardial infarction. Heart. 2020;106:24-32.

LinkOut - more resources