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. 2021 May 18;11(1):10530.
doi: 10.1038/s41598-021-89929-3.

Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population

Oyetunde Timothy Oyeyemi  1   2 , Camila Amormino Corsini  3 , Gustavo Gonçalves  4 , William de Castro Borges  4 , Rafaella Fortini Queiroz Grenfell  5
Affiliations

Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population

Oyetunde Timothy Oyeyemi et al. Sci Rep. .

Abstract

The study aimed to determine the potential of schistosomula crude antigen (SCA) as a diagnostic target for anti-S. mansoni antibody detection. Cercariae were transformed into schistosomula, homogenized through sonication, and then centrifuged to obtain the SCA. SCA was evaluated using ELISA and dot blots immunoassays on 30 S. mansoni infected sera samples obtained from chronic patients and 30 non-infected humans' sera samples. Either Kato-Katz or saline gradient method or both were employed as the diagnostic reference. Dot blots immunoassay was further performed on protein eluted from 10 to 12 kDa immunoreactive band identified by Western blot analysis. The area under the ROC curve was 0.95 (AUC 0.95, CI 0.88-1.01, p < 0.0001). The sensitivity and specificity of SCA-ELISA and dot blots assays were 96.67% and 86.67% respectively. The human IgG-specific response against SCA was significantly higher in S. mansoni infected individuals (OD = 0.678 ± 0.249) compared to the non-infected population (OD = 0.235 ± 0.136) (p < 0.0001). Our study showed that SCA and its 10-12 kDa component could be useful as diagnostic tools for chronic schistosomiasis.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Western blot analysis of SCA and S. mansoni positive and negative sera.
Figure 2
Figure 2
Receiver operating characteristic curve of ELISA immunoassay of SCA.
Figure 3
Figure 3
Antibody production in relation to S. mansoni infection status.
Figure 4
Figure 4
Dot blot assays of SCA (A) and immunoreactive protein band (10–12 kDa) (B).
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References

    1. World Health Organization (WHO). Schistosomiasis. https://www.who.int/en/news-room/fact-sheets/detail/schistosomiasis. Accessed 27 May 2020.
    1. Santos MB, et al. Spatial analysis of viral hepatitis and schistosomiasis coinfection in an endemic area in Northeastern Brazil. Rev. Soc. Bras. Med. Trop. 2017;50:383–387. doi: 10.1590/0037-8682-0411-2016. - DOI - PubMed
    1. Brasil, Ministério da Saúde, Secretaria de Vigilância em Saúde, Departamento de Vigilância das Doenças Transmissíveis. Vigilância da esquistossomose mansönica: diretrizes técnicas, 4a. ed. (Ministério da Saúde, 2014).
    1. Salawu OT, Odaibo AB. Urogenital schistosomiasis and urological assessment of hematuria in preschool-aged children in rural communities of Nigeria. J. Ped. Urol. 2014;10:88–93. doi: 10.1016/j.jpurol.2013.06.010. - DOI - PubMed
    1. Ebrahim A, El-Morshedy H, Omer E, El-Daly S, Barakat R. Evaluation of the Kato-Katz thick smear and formol ether sedimentation techniques for quantitative diagnosis of Schistosoma mansoni infection. Am. J. Trop. Med. Hyg. 1997;57:706–708. doi: 10.4269/ajtmh.1997.57.706. - DOI - PubMed

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