Comparative Study
Application of biotin-avidin system, determination of circulating immune complexes, and evaluation of antibody response in different hydatidosis patients
Affiliations
- PMID: 3400804
Item in Clipboard
Comparative Study
Application of biotin-avidin system, determination of circulating immune complexes, and evaluation of antibody response in different hydatidosis patients
Am J Trop Med Hyg.
1988 Jul.
Abstract
The avidin-biotin-peroxidase complex enzyme-linked immunosorbent assay (ABC-ELISA) and standard ELISA were used for the detection of Echinococcus granulosus antibody in sera of 101 patients operated on for hydatid disease, 40 patients with miscellaneous nonhydatid diseases, and 61 normal subjects. Sensitivity and specificity of the two procedures were comparable and the geometric mean antibody titer detected with ABC-ELISA was higher than with standard ELISA. The ABC-ELISA is a sensitive, specific, simple, and convenient method for diagnosing hydatidosis.
Similar articles
-
[Avidin-biotin-peroxidase complex enzyme-linked immunosorbent assay in the diagnosis of human multilocular echinococcosis].Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1989;7(3):197-200. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1989. PMID: 2591036 Chinese.
-
Use of two polysaccharide antigens in ELISA for the detection of antibodies to Echinococcus granulosus in sheep sera.Res Vet Sci. 1987 Sep;43(2):257-63. Res Vet Sci. 1987. PMID: 3685640
-
[ABC-ELISA in the detection of specific IgE in patients with hydatidosis].Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1988;6(4):253-5. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1988. PMID: 3253023 Chinese. No abstract available.
-
[Serology of hydatidosis].Enferm Infecc Microbiol Clin. 1991 Apr;9(4):237-47. Enferm Infecc Microbiol Clin. 1991. PMID: 1863624 Review. Spanish. No abstract available.
-
Echinococcus granulosus: immunodiagnosis and vaccination, a perspective.Parassitologia. 1997 Dec;39(4):345-7. Parassitologia. 1997. PMID: 9802090 Review.
Cited by
-
Tropical medicine.Postgrad Med J. 1991 Sep;67(791):798-822. doi: 10.1136/pgmj.67.791.798. Postgrad Med J. 1991. PMID: 1946126 Free PMC article. Review. No abstract available.
-
Immunological diagnosis of human hydatid cyst relapse: utility of the enzyme-linked immunoelectrotransfer blot and discriminant analysis.Clin Diagn Lab Immunol. 2000 Jul;7(4):549-52. doi: 10.1128/CDLI.7.4.549-552.2000. Clin Diagn Lab Immunol. 2000. PMID: 10882649 Free PMC article.