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. 1988 May:25 ( Pt 3):319-24.
doi: 10.1177/000456328802500322.

Analysis of proteinuria using a commercial system for automated electrophoresis and isoelectric focusing

P J Jackson  1 , C J Sampson, E H Cooper, D Heney, J T Brocklebank
Affiliations

Analysis of proteinuria using a commercial system for automated electrophoresis and isoelectric focusing

P J Jackson et al. Ann Clin Biochem. 1988 May.

Abstract

We describe an investigation of proteinuria using Pharmacia PhastSystemTM electrophoresis apparatus. The analysis of urinary proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of unconcentrated urine followed by silver staining took about 2 h and could clearly demonstrate tubular dysfunction or glomerular damage in urines with a negative or only trace-positive dip-stick test for protein. In addition, we show the identification of urinary proteins by immunoblotting from SDS-PAGE gels and the characterisation of Bence-Jones proteins by isoelectric focusing (IEF) and immunoblotting.

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