Study by absorption and emission spectrophotometry of the efficiency of the binary mixture (Ethanol-Water) on the extraction of betanin from red beetroot

Abstract

In this paper, we intend to present a classical method of extraction and analysis of betanin from Algerian red beetroot, and to know the efficiency of the binary mixture (Ethanol-Water) on the extraction. Also, to know the betanin content of the extract obtained using optical spectroscopic methods. This dye is a natural pigment with high antioxidant power and widely used as a natural dye. In addition, the high bioavailability of this pigment reinforces its potential for future therapeutic applications. However, commercially the betanin content beet extracts never exceeds 1,2% and this molecule does not exist marketed in a more purified form, therefore it is necessary to have efficient tools for the enrichment of betanin extracts. The experimental results made in our laboratory, confirm the effectiveness of the binary mixture (Ethanol-Water) on the betanin extraction from Algerian beetroot, the yield obtained of extracted betanin pigment from red beetroot is 0,342 mg/g, when the mixture of solvents (Ethanol-Water) is used with a large quantity of water. As well as, the betanin has an absorption band varying from 480 nm to 540 nm and it has an emission band at 800 nm.

Keywords: Absorption and emission spectrophotometry; Betanin; Extraction analysis; Red beetroot.