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. 2021 Jul 5:561:52-58.
doi: 10.1016/j.bbrc.2021.05.019. Epub 2021 May 18.

Molecular characterization and functional analysis of duck CCCH-type zinc finger antiviral protein (ZAP)

Rongrong Zhang  1 , Yan He  2 , Xinyu Zhu  3 , Guoyuan Wen  2 , Qingping Luo  2 , Tengfei Zhang  2 , Qin Lu  2 , Shudan Liu  3 , Shaobo Xiao  3 , Liurong Fang  4 , Huabin Shao  5
Affiliations

Molecular characterization and functional analysis of duck CCCH-type zinc finger antiviral protein (ZAP)

Rongrong Zhang et al. Biochem Biophys Res Commun. .

Abstract

This is the first study to clone duck CCCH-type zinc finger antiviral protein (duZAP) from Jingjiang duck (Anas platyrhynchos). Full-length duZAP cDNA was 2154 bp and encoded a 717-amino acid polypeptide containing four highly conserved CCCH-type finger motifs, a WWE domain and a poly (ADP-ribose) polymerase (PARP) domain. duZAP was expressed in multiple duck tissues, with the highest mRNA expression in the spleen. Overexpression of duZAP in duck embryo fibroblast cells (DEFs) led to activation of the transcription factors IRF1 and NF-κB, and induction of IFN-β. Analysis of deletion mutants revealed that both the WWE and PARP domains of duZAP were essential for activating the IFN-β promoter. Knockdown of duZAP in DEFs significantly reduced poly (I:C)- and duck Tembusu virus (DTMUV)-induced IFN-β activation. Our findings further the understanding of the role of duZAP in the duck innate immune response.

Keywords: DTMUV; Duck; Interferon-β; ZAP.

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Conflict of interest statement

Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationship that could be construed as a potential conflict of interest.

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