Bringing Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging to the Clinics

Sankha S Basu¹, Nathalie Y R Agar²

Affiliations

¹ Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
² Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. Electronic address: Nathalie_Agar@dfci.harvard.edu.

PMID: 34020766
PMCID: PMC8604036
DOI: 10.1016/j.cll.2021.03.009

Review

Bringing Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging to the Clinics

Sankha S Basu et al. Clin Lab Med. 2021 Jun.

. 2021 Jun;41(2):309-324.

doi: 10.1016/j.cll.2021.03.009.

Authors

Sankha S Basu¹, Nathalie Y R Agar²

Affiliations

¹ Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
² Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. Electronic address: Nathalie_Agar@dfci.harvard.edu.

PMID: 34020766
PMCID: PMC8604036
DOI: 10.1016/j.cll.2021.03.009

Abstract

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is an emerging analytical technique that promises to change tissue-based diagnostics. This article provides a brief introduction to MALDI MSI as well as clinical diagnostic workflows and opportunities to apply this powerful approach. It describes various MALDI MSI applications, from more clinically mature applications such as cancer to emerging applications such as infectious diseases and drug distribution. In addition, it discusses the analytical considerations that need to be considered when bringing these approaches to different diagnostic problems and settings.

Keywords: Cancer; Clinical; Drug distribution; Infectious disease; MALDI; Mass spectrometry imaging.

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References

1. Buchberger AR, DeLaney K, Johnson J, et al. Mass spectrometry imaging: a review of emerging advancements and future insights. Anal Chem 2018;90(1):240–65. - PMC - PubMed
1. Ferguson CN, Fowler JWM, Waxer JF, et al. Mass spectrometry-based tissue imaging of small molecules. Adv Exp Med Biol 2019;1140:99–109. - PubMed
1. Gode D, Volmer DA. Lipid imaging by mass spectrometry – a review. Analyst 2013. 10.1039/c2an36337b. - DOI - PubMed
1. Gessel MM, Norris JL, Caprioli RM. MALDI imaging mass spectrometry: spatial molecular analysis to enable a new age of discovery. J Proteomics 2014;107:71–82. 10.1016/j.jprot.2014.03.021. - DOI - PMC - PubMed
1. Zhang J, Sans M, Garza KY, et al. Mass spectrometry technologies to advance care for cancer patients IN clinical and intraoperative use. Mass Spectrom Rev 2020. 10.1002/mas.21664. - DOI - PubMed

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Bringing Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging to the Clinics

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Bringing Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging to the Clinics

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