Localization and characterization of human papillomavirus-16 in oral squamous cell carcinoma

doi:10.1111/odi.13920

. 2023 Mar;29(2):436-444.

doi: 10.1111/odi.13920. Epub 2021 Jun 21.

Localization and characterization of human papillomavirus-16 in oral squamous cell carcinoma

Wafaa Saleh^{1

2}, Seunghee Cha¹, Abdulaziz Banasser¹, Sarah G Fitzpatrick¹, Indraneel Bhattacharyya¹, Jilan M Youssef², Mohamed M Anees², Islam A Elzahaby³, Joseph Katz¹

Affiliations

¹ Oral Diagnostic Sciences Department, College of Dentistry, University of Florida, Gainesville, FL, USA.
² Oral Medicine and Periodontology Department, Faculty of Dentistry, Mansoura University, Mansoura, FL, USA.
³ Department of Surgical Oncology, Mansoura Oncology Center, Mansoura University, Mansoura, FL, USA.

PMID: 34022097
DOI: 10.1111/odi.13920

Localization and characterization of human papillomavirus-16 in oral squamous cell carcinoma

Wafaa Saleh et al. Oral Dis. 2023 Mar.

. 2023 Mar;29(2):436-444.

doi: 10.1111/odi.13920. Epub 2021 Jun 21.

Authors

Wafaa Saleh^{1

2}, Seunghee Cha¹, Abdulaziz Banasser¹, Sarah G Fitzpatrick¹, Indraneel Bhattacharyya¹, Jilan M Youssef², Mohamed M Anees², Islam A Elzahaby³, Joseph Katz¹

Affiliations

¹ Oral Diagnostic Sciences Department, College of Dentistry, University of Florida, Gainesville, FL, USA.
² Oral Medicine and Periodontology Department, Faculty of Dentistry, Mansoura University, Mansoura, FL, USA.
³ Department of Surgical Oncology, Mansoura Oncology Center, Mansoura University, Mansoura, FL, USA.

PMID: 34022097
DOI: 10.1111/odi.13920

Abstract

Objectives: The role of Human papillomavirus (HPV) in the oral squamous cell carcinoma (OSCC) has not been completely elucidated. The purpose of the present study was to investigate the prevalence and localization of HPV-16 virus in OSCC and to correlate HPV-16 positivity and p16INK4A expression with the clinical and pathological features of OSCC.

Methods: The archives of Oral Pathology at the University of Florida, College of Dentistry were accessed for demographic, clinical, histopathological data, and slides of 114 OSCC patients. HPV-16 positivity of OSCC was evaluated by p16INK4A immunohistochemistry (IHC) and HPV-16 E6/E7mRNA by in situ hybridization (ISH).

Results: Out of 114 consecutive pathological slides of OSCC, 16 samples (14%) showed positivity for p16INK4A by IHC and 14 samples (12%) were positive for HPV-16 E6/E7mRNA ISH and the Positivity showed a significant correlation with the patients' age, alcohol consumption, and the degree of OSSC differentiation. The hard palate showed the highest positivity of p16INK4A IHC and HPV-16 mRNA ISH (38%, 36% respectively).

Conclusion: HPV-16 is a significant factor in oral carcinogenesis. We recommend using p16INK4A as a surrogate marker for HPV detection in OSCC, which can be complemented by RNA ISH for the identification of HPV subtypes.

Keywords: human papillomavirus; immunohistochemistry; in situ hybridization; oral squamous cell carcinoma; p16INK4A.

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References

REFERENCES

1. Abreu, P. M., Valle, I. B., Damasceno, T. C. D., Có, A. C. G., Pansini, P. F., Podestá, J. R. V., Souza, E. D., Rocha, R. M., Curado, M. P., Mehanna, H., & von Zeidler, S. V. (2020). Human Papillomavirus E6/E7 mRNA detection by in situ hybridization in oral cavity squamous cell carcinoma. Archives of Oral Biology, 116, 104746-https://doi.org/10.1016/j.archoralbio.2020.104746
1. Belobrov, S., Angel, C., Wiesenfeld, D., & McCullough, M. (2016). Histopathological analysis of oral squamous cell carcinoma in nonsmokers and nondrinkers. Translational Research in Oral Oncology, 1, 1-10. https://doi.org/10.1177/2057178X16647973
1. Belobrov, S., Cornall, A. M., Young, R. J., Koo, K., Angel, C., Wiesenfeld, D., Rischin, D., Garland, S. M., & McCullough, M. (2018). The role of human papillomavirus in p16-positive oral cancers. Journal of Oral Pathology and Medicine, 47(1), 18-24. https://doi.org/10.1111/jop.12649
1. Bishop, J. A., Ma, X.-J., Wang, H., Luo, Y., Illei, P. B., Begum, S., Taube, J. M., Koch, W. M., & Westra, W. H. (2012). Detection of transcriptionally active high-risk HPV in patients with head and neck squamous cell carcinoma as visualized by a novel E6/E7 mRNA in situ hybridization method. American Journal of Surgical Pathology, 36(12), 1874-1882. https://doi.org/10.1097/PAS.0b013e318265fb2b
1. Bussu, F., Sali, M., Gallus, R., Vellone, V. G., Zannoni, G. F., Autorino, R., Dinapoli, N., Santangelo, R., Martucci, R., Graziani, C., Miccichè, F., Almadori, G., Galli, J., Delogu, G., Sanguinetti, M., Rindi, G., Valentini, V., & Paludetti, G. (2013). HPV infection in squamous cell carcinomas arising from different mucosal sites of the head and neck region. Is p16 immunohistochemistry a reliable surrogate marker? British Journal of Cancer, 108(5), 1157-1162. https://doi.org/10.1038/bjc.2013.55

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JS-3785/This study was funded by the grant (JS-3785) supported by the Egyptian Cultural and Educational Bureau in Washington DC, Egyptian Mission sector and Ministry of Higher Education in Egypt.

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[1] Abreu, P. M., Valle, I. B., Damasceno, T. C. D., Có, A. C. G., Pansini, P. F., Podestá, J. R. V., Souza, E. D., Rocha, R. M., Curado, M. P., Mehanna, H., & von Zeidler, S. V. (2020). Human Papillomavirus E6/E7 mRNA detection by in situ hybridization in oral cavity squamous cell carcinoma. Archives of Oral Biology, 116, 104746-https://doi.org/10.1016/j.archoralbio.2020.104746

[2] Abreu, P. M., Valle, I. B., Damasceno, T. C. D., Có, A. C. G., Pansini, P. F., Podestá, J. R. V., Souza, E. D., Rocha, R. M., Curado, M. P., Mehanna, H., & von Zeidler, S. V. (2020). Human Papillomavirus E6/E7 mRNA detection by in situ hybridization in oral cavity squamous cell carcinoma. Archives of Oral Biology, 116, 104746-https://doi.org/10.1016/j.archoralbio.2020.104746

[3] Belobrov, S., Angel, C., Wiesenfeld, D., & McCullough, M. (2016). Histopathological analysis of oral squamous cell carcinoma in nonsmokers and nondrinkers. Translational Research in Oral Oncology, 1, 1-10. https://doi.org/10.1177/2057178X16647973

[4] Belobrov, S., Angel, C., Wiesenfeld, D., & McCullough, M. (2016). Histopathological analysis of oral squamous cell carcinoma in nonsmokers and nondrinkers. Translational Research in Oral Oncology, 1, 1-10. https://doi.org/10.1177/2057178X16647973

[5] Belobrov, S., Cornall, A. M., Young, R. J., Koo, K., Angel, C., Wiesenfeld, D., Rischin, D., Garland, S. M., & McCullough, M. (2018). The role of human papillomavirus in p16-positive oral cancers. Journal of Oral Pathology and Medicine, 47(1), 18-24. https://doi.org/10.1111/jop.12649

[6] Belobrov, S., Cornall, A. M., Young, R. J., Koo, K., Angel, C., Wiesenfeld, D., Rischin, D., Garland, S. M., & McCullough, M. (2018). The role of human papillomavirus in p16-positive oral cancers. Journal of Oral Pathology and Medicine, 47(1), 18-24. https://doi.org/10.1111/jop.12649

[7] Bishop, J. A., Ma, X.-J., Wang, H., Luo, Y., Illei, P. B., Begum, S., Taube, J. M., Koch, W. M., & Westra, W. H. (2012). Detection of transcriptionally active high-risk HPV in patients with head and neck squamous cell carcinoma as visualized by a novel E6/E7 mRNA in situ hybridization method. American Journal of Surgical Pathology, 36(12), 1874-1882. https://doi.org/10.1097/PAS.0b013e318265fb2b

[8] Bishop, J. A., Ma, X.-J., Wang, H., Luo, Y., Illei, P. B., Begum, S., Taube, J. M., Koch, W. M., & Westra, W. H. (2012). Detection of transcriptionally active high-risk HPV in patients with head and neck squamous cell carcinoma as visualized by a novel E6/E7 mRNA in situ hybridization method. American Journal of Surgical Pathology, 36(12), 1874-1882. https://doi.org/10.1097/PAS.0b013e318265fb2b

[9] Bussu, F., Sali, M., Gallus, R., Vellone, V. G., Zannoni, G. F., Autorino, R., Dinapoli, N., Santangelo, R., Martucci, R., Graziani, C., Miccichè, F., Almadori, G., Galli, J., Delogu, G., Sanguinetti, M., Rindi, G., Valentini, V., & Paludetti, G. (2013). HPV infection in squamous cell carcinomas arising from different mucosal sites of the head and neck region. Is p16 immunohistochemistry a reliable surrogate marker? British Journal of Cancer, 108(5), 1157-1162. https://doi.org/10.1038/bjc.2013.55

[10] Bussu, F., Sali, M., Gallus, R., Vellone, V. G., Zannoni, G. F., Autorino, R., Dinapoli, N., Santangelo, R., Martucci, R., Graziani, C., Miccichè, F., Almadori, G., Galli, J., Delogu, G., Sanguinetti, M., Rindi, G., Valentini, V., & Paludetti, G. (2013). HPV infection in squamous cell carcinomas arising from different mucosal sites of the head and neck region. Is p16 immunohistochemistry a reliable surrogate marker? British Journal of Cancer, 108(5), 1157-1162. https://doi.org/10.1038/bjc.2013.55

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Localization and characterization of human papillomavirus-16 in oral squamous cell carcinoma

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Localization and characterization of human papillomavirus-16 in oral squamous cell carcinoma

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