The role of microRNAs in epithelial to mesenchymal transition and cancers; focusing on mir-200 family

Abstract

Epithelial to mesenchymal transition (EMT) is a process associated with cancer malignancy and metastases. Cells undergoing EMT lose their epithelial phenotype and acquire mesenchymal phenotype. This process is accompanied by several molecular changes such as decrease of E-cadherin and increase of N-cadherin which is called the "cadherin swich". MicroRNAs (miRNAs, miRs) are small non-coding RNAs having ability to regulate genes post-transcriptionally. Nowadays they are believed to take part in multiple physiological and pathological processes including cancer development. Comparison between TargetScan7 (www.targetscan.org) results for miR-200b and metanalysis of genes involved in EMT showed that miR-200b has a potential binding site in 60 genes that are involved in EMT (the majority of them were associated with mesenchymal phenotype). Our review summarizes literature findings contributing to experimentally proven interactions between miR-200b and genes involved in EMT process including cell receptors, signaling pathways, cell cycle or cell adhesion. The results of those interactions indicate that miR-200b may have an inhibitory impact on EMT or even in selected cases is able to restore epithelial phenotype.

Keywords: Cancers; EMT; Epithelial to mesenchymal transition; miR-200b; microRNA.