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. 2019 Jul 22;17(3):613-620.
doi: 10.5114/aoms.2019.86770. eCollection 2021.

The association of endothelin-1 gene polymorphism and its plasma levels with hypertension and coronary atherosclerosis

Nazanin Ebrahimi  1 , Gholamreza Asadikaram  2 , Abbas Mohammadi  3 , Yunes Jahani  4   5 , Mina Moridi  6 , Mohammad Masoumi  7
Affiliations

The association of endothelin-1 gene polymorphism and its plasma levels with hypertension and coronary atherosclerosis

Nazanin Ebrahimi et al. Arch Med Sci. .

Abstract

Introduction: Endothelin-1 (ET-1) is the most potent among all vasoconstrictors, and its association with cardiovascular diseases has been reported before. Our aim was to investigate the association of ET-1 plasma level and its gene polymorphisms (rs5370 and rs10478694) with hypertension and coronary atherosclerosis (CA).

Material and methods: This study was carried out on 128 women and 132 men, who were divided into 4 groups: hypertensive without atherosclerosis (H+A-); hypertensive with atherosclerosis (H+A+); non-hypertensive with atherosclerosis (H-A+); and non-hypertensive without atherosclerosis (control group). Endothelin-1 plasma levels were measured by ELISA, and gene polymorphisms were detected by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) methods. Coronary artery diseases (CAD) were then defined based on angiography data.

Results: The ET-1 plasma level was significantly higher in the H+A+ group in comparison with the other groups, especially H+A-. Comparing people with CA and those without it, the highest frequency level of the T allele of rs5370 was found in people with CA. Significantly higher frequencies of the 3A allele were detected in hypertensive patients in comparison with non-hypertensive individuals, when analyzing rs10478694.

Conclusions: Endothelin-1 plasma level shows a direct association with the risk of CA development. The T allele of rs5370 can be regarded as a risk factor for CA development. The 3A allele of rs10478694 can be associated with the risk of hypertension; therefore, it can be concluded that ET-1 and its gene polymorphisms play an important role in CA development and hypertension observed in the south-eastern populations of Iran.

Keywords: SNP; atherosclerosis; endothelin-1; hypertension; rs1046872; rs5370.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Comparison of endothelin-1 plasma level among the four groups under investigation Endothelin-1 plasma level in the H+A+ group is significantly higher than in the other groups (p = 0.018). Only the difference of endothelin-1 plasma level observed between H+A+ and H+A– is significant (p = 0.017). H+A– – hypertension and no coronary artery disease, H–A+ – coronary artery disease and no hypertension, H+A+ – coronary artery disease and hypertension, H–A– – no coronary artery disease and no hypertension. Significant difference at < 0.05
See this image and copyright information in PMC

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