Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021:2299:323-338.
doi: 10.1007/978-1-0716-1382-5_22.

Mouse Models of Kidney Fibrosis

Rafael Kramann  1   2 , Sylvia Menzel  3
Affiliations

Mouse Models of Kidney Fibrosis

Rafael Kramann et al. Methods Mol Biol. 2021.

Abstract

Chronic kidney disease (CKD) affects over 10% of the worldwide population and kidney fibrosis is a main driver of CKD and considered a therapeutic target. The mechanisms leading to kidney fibrosis are highly complexed and can be best studied in rodent models. Here we describe the most commonly used kidney fibrosis models in mice, the unilateral ureteral obstruction (UUO) model and the ischemia reperfusion injury (IRI) model. Both models are easy to learn and can be applied in animals of different age, sex, and strain.

Keywords: Hydronephrosis; IRI; Mouse model; UUO; Unilateral ischemia reperfusion.

PubMed Disclaimer

References

    1. Kramann R, Machado F, Wu H, Kusaba T, Hoeft K, Schneider RK, Humphreys BD (2018) Parabiosis and single-cell RNA sequencing reveal a limited contribution of monocytes to myofibroblasts in kidney fibrosis. JCI Insight 3(9):e99561. https://doi.org/10.1172/jci.insight.99561 - DOI - PMC
    1. Kramann R, Wongboonsin J, Chang-Panesso M, Machado FG, Humphreys BD (2017) Gli1+ pericyte loss induces capillary rarefaction and proximal tubular injury. J Am Soc Nephrol 28(3):776–784 - DOI
    1. Rabe M, Schaefer F (2016) Non-transgenic mouse models of kidney disease. Nephron 133(1):53–61. https://doi.org/10.1159/000445171 - DOI - PubMed
    1. Martínez-Klimova E, Aparicio-Trejo OE, Tapia E, Pedraza-Chaverri J (2019) Unilateral ureteral obstruction as a model to investigate fibrosis-attenuating treatments. Biomol Ther 9(4):141. https://doi.org/10.3390/biom9040141 - DOI
    1. Heung M, Chawla LS (2014) Acute kidney injury: gateway to chronic kidney disease. Nephron Clin Pract 127(1–4):30–34. https://doi.org/10.1159/000363675 - DOI - PubMed

LinkOut - more resources