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. 2021 Jun;35(6):e23770.
doi: 10.1002/jcla.23770. Epub 2021 May 24.

Serum HDL cholesterol uptake capacity in subjects from the MASHAD cohort study: Its value in determining the risk of cardiovascular endpoints

Malihe Aghasizadeh  1   2 , Sara Samadi  3 , Amirhossein Sahebkar  4 , Ebrahim Miri-Moghaddam  2 , Habibollah Esmaily  5 , Mohamad Souktanloo  6 , Amir Avan  7   8 , Amin Mansoori  9 , Gordon A Ferns  10 , Tooba Kazemi  2   11 , Majid Ghayour-Mobarhan  12
Affiliations

Serum HDL cholesterol uptake capacity in subjects from the MASHAD cohort study: Its value in determining the risk of cardiovascular endpoints

Malihe Aghasizadeh et al. J Clin Lab Anal. 2021 Jun.

Abstract

Background: The efficiency of high-density lipoprotein (HDL) to efflux cholesterol contributes to the reverse cholesterol transport (RCT) pathway as one of HDL's proposed functions and depends on the ability of HDL to uptake cholesterol. We aimed to investigate cholesterol uptake capacity (CUC) by a newly developed assay in samples from the MASHAD (Mashhad Stroke and Heart Atherosclerotic Disorders) cohort study.

Method: The study population comprised 153 individuals developed CVD diagnosed by a specialist cardiologist, over 6 years of follow-up, and 350 subjects without CVD. We used a modified CUC method to evaluate the functionality of HDL in serum samples.

Result: The CUC assay was highly reproducible with values for inter- and intra-assay variation of 13.07 and 6.65, respectively. The mean serum CUC was significantly lower in the CVD group compared to control (p = 0.01). Although, there were no significant differences in serum HDL-C between the groups and there was no significantly association with risk of progressive CVD. Multivariate logistic regression analysis showed that there was a significantly negative association between CUC and risk of CVD after adjustment for confounding parameters (OR = 0.57, 95% CI = 0.38-0.87, p = 0.009). The CUC was also inversely and independently associated with the risk of CVD event using Cox proportional hazards models analysis (HR = 0.62; 95% CI = 0.41-0.94, p = 0.02). We determined the optimum cutoff value of 1.7 a.u for CUC in the population. Furthermore, the CUC value was important in determining the CVD risk stratification derived from data mining analysis.

Conclusions: Reduced HDL functionality, as measured by CUC, appears to predict CVD in population sample from north-eastern Iran.

Keywords: HDL function; cardiovascular disease (CVD); cholesterol uptake capacity (CUC); cohort study.

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Conflict of interest statement

The authors have no conflict of interest to disclose.

Figures

FIGURE 1
FIGURE 1
Box‐plots of high‐density lipoprotein (HDL‐C) (A) and cholesterol uptake capacity (CUC) (B) in the MASHAD cohort study population comprising 350 healthy individuals without clinical cardiovascular disease and 153 subjects without CVD at baseline who then developed CVD outcomes over 6 years of follow‐up. a.u.; Arbitrary unit
FIGURE 2
FIGURE 2
Kaplan‐Meier curve and hazard ratios for CUC cutoff point, derived from Cox proportional hazards models (Log Rank = 0.003). The hazard ratio was adjusted for sex, age, smoking status, BMI, total cholesterol level, diabetes, and hypertension. CUC: cholesterol uptake capacity
FIGURE 3
FIGURE 3
Decision tree for cardiovascular event in MASHAD cohort study (The groups with and without incident CVD events represent as blue and red, respectively)
FIGURE 4
FIGURE 4
Scatterplot Matrix, Multivariate platform options derived from data mining, The correlation between lipid profile (LDL, HDL, TC, and TG) and cholesterol uptake capacity or CUC value
See this image and copyright information in PMC

References

    1. Robins SJ, Collins D, Wittes JT, et al. Relation of gemfibrozil treatment and lipid levels with major coronary events: VA‐HIT: a randomized controlled trial. JAMA. 2001;285(12):1585‐1591. - PubMed
    1. Rosenson RS, Brewer HB Jr, Davidson WS, et al. Cholesterol efflux and atheroprotection: advancing the concept of reverse cholesterol transport. Circulation. 2012;125(15):1905‐1919. - PMC - PubMed
    1. Reiner Ž. Hypertriglyceridaemia and risk of coronary artery disease. Nat Rev Cardiol. 2017;14(7):401. - PubMed
    1. Voight BF, Peloso GM, Orho‐Melander M, et al. Plasma HDL cholesterol and risk of myocardial infarction: a mendelian randomisation study. Lancet. 2012;380(9841):572‐580. - PMC - PubMed
    1. Lincoff AM, Nicholls SJ, Riesmeyer JS, et al. Evacetrapib and cardiovascular outcomes in high‐risk vascular disease. N Engl J Med. 2017;376(20):1933‐1942. - PubMed