Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Sep;101(9):725-736.
doi: 10.1002/cyto.a.24461. Epub 2021 May 24.

Fluorescence activated cell sorting-A selective tool for plant cell isolation and analysis

Ioanna Antoniadi  1 , Vladimír Skalický  2 , Guiling Sun  3 , Wen Ma  3 , David W Galbraith  4   3 , Ondřej Novák  1   2 , Karin Ljung  1
Affiliations
Free article
Review

Fluorescence activated cell sorting-A selective tool for plant cell isolation and analysis

Ioanna Antoniadi et al. Cytometry A. 2022 Sep.
Free article

Abstract

Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single-cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single-cell suspensions can be obtained through digestion of the cells walls and release of the so-called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed.

Keywords: autofluorescence; best practices; plant flow cytometry and sorting; protoplasts; viability and integrity.

PubMed Disclaimer

References

REFERENCES

    1. Cocking EC. A method for the isolation of plant protoplasts and vacuoles. Nature. 1960;187:962-3.
    1. Mazarei M, Al-Ahmad H, Rudis MR, Stewart CN Jr. Protoplast isolation and transient gene expression in switchgrass, Panicum virgatum L. Biotechnol J. 2008;3:354-9.
    1. Priyadarshani SVGN, Hu B, Li W, Ali H, Jia H, Zhao L, et al. Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.). Plant Methods. 2018;14:95.
    1. Yoo SD, Cho YH, Sheen J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc. 2007;2:1565-72.
    1. Davey MR, Anthony P, Power JB, Lowe KC. Plant protoplasts: status and biotechnological perspectives. Biotechnol Adv. 2005;23:131-71.

Publication types

LinkOut - more resources