Ganoderic Acid A Alleviates OVA-Induced Asthma in Mice

Abstract

The aim of this study is to investigate the effects of ganoderic acid A (GAA) on OVA-induced asthma in mice. Mouse asthma model was established by ovalbumin (OVA) in vitro. Diff-Quik staining was used to observe the total numbers of cells and the number of classification cells in each group, and HE staining was used to observe lung inflammation in lung tissue sections. ELISA was used to detect the effect of GAA on the levels of interleukin-4 (IL-4), IL-5, and IL-13 in serum and lung tissue. The expression levels of TLR/NF-κB were detected by Western blot. Immunohistochemistry was used to observe the expression changes of TLR4 and P-P65. Compared with the normal group, the inflammatory cell count, IL-4, IL-5, and IL-13 expression in the model group increased, and TLR/NF-kB signal protein expression increased. Compared with the model group, in GAA group, the number of inflammatory cells, the expression of IL-4, IL-5, and IL-13 decreased, and the expression of TLR/NF-kB signaling protein decreased. GAA regulated lung inflammation in asthmatic mice by inhibiting TLR/NF-kB signaling pathway.

Keywords: TLR/NF-kB; asthma; ganoderic acid A; inflammation.

Fig. 1

GAA attenuated cellular changes in serum of asthmatic mice. Values are expressed as means ± SEM. Compared with control: ^#P < 0.05, ^##P < 0.01; compared with OVA:*P < 0.05, **P < 0.01.

Fig. 2

Pathological changes. Values are expressed as means ± SEM. Compared with control: ^#P < 0.05, ^##P < 0.01; compared with OVA:*P < 0.05, **P < 0.01.

Fig. 3

IL-4, IL-5, and IL-13 levels in serum of asthmatic mice. Values are expressed as means ± SEM. Compared with control: ^#P < 0.05, ^##P < 0.01; compared with OVA:*P < 0.05, **P < 0.01.

Fig. 4

AHR in asthmatic mice. Values are expressed as means ± SEM. Compared with control: ^#P < 0.05, ^##P < 0.01; compared with OVA:*P < 0.05, **P < 0.01.

Fig. 5

Effect of GAA on IgE in serum. Values are expressed as means ± SEM. Compared with control: ^#P < 0.05, ^##P < 0.01; compared with OVA:*P < 0.05, **P < 0.01.

Fig. 6

Effect of GAA on TLR/NF-kB signaling pathway in the lung. Values are expressed as means ± SEM. Compared with control: ^#P < 0.05, ^##P < 0.01; compared with OVA:*P < 0.05, **P < 0.01.

Fig. 7

Effect of GAA on TLR4 and p-NF-kBp65 in the lung. Values are expressed as means ± SEM. Compared with control: ^#P < 0.05, ^##P < 0.01; compared with OVA:*P < 0.05, **P < 0.01.