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. 2021 May 18;7(5):e07065.
doi: 10.1016/j.heliyon.2021.e07065. eCollection 2021 May.

Determination of singlet oxygen quenching rate and mechanism of γ-oryzanol

Yuli Perwita Sari  1 , Umar Santoso  1 , Supriyadi  1 , Sri Raharjo  1
Affiliations

Determination of singlet oxygen quenching rate and mechanism of γ-oryzanol

Yuli Perwita Sari et al. Heliyon. .

Abstract

Photooxidation is one of the causes of quality deterioration in food. An antioxidant or singlet oxygen quencher is urgently needed to prevent photooxidation. γ-Oryzanol was recognized as a naturally present antioxidant in rice bran products. This research aimed to calculate the singlet oxygen quenching rate and its mechanism of γ-oryzanol to evaluate the potency of γ-oryzanol as singlet oxygen quencher. A series of linoleic acid (50 and 100 mM) or γ-oryzanol (0.7 and 1.5 mM) were prepared separately in ethanol: chloroform (96:4, v/v) containing 25 ppm of erythrosine. High-Performance Liquid Chromatography quantified the degradation of γ-oryzanol. Meanwhile, Gas Chromatography determined the changes in linoleic acid content during photooxidation. The singlet oxygen quenching rate was calculated by steady-state. The singlet oxygen quenching rate of γ-oryzanol was 3.04 × 106/M/s by physical and chemical quenching mechanism. Photooxidation caused the declined of γ-oryzanol by 0.1421 mM/h. Based on singlet oxygen quenching rate calculation, it suggests that γ-oryzanol can perform as a singlet oxygen quencher with slightly dominated by physical quenching mechanism (52.28%). The rest it performed via a chemical quenching mechanism.

Keywords: Nanoemulsion; Photooxidation; Rice bran oil; Singlet oxygen quenching; γ-Oryzanol.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The illustration of light storage box. The lamps were Philips Lifemax (TLD 18W/840 Cool White, TIS 956–2533|TIS 236–2533).
Figure 2
Figure 2
Three parameters of photo-oxidation products of linoleic acid under illumination at 3400 lux: peroxide value (a), anisidine value (b) and TOTOX value (c).
Figure 3
Figure 3
Effect of light 3400 lux and sensitizer (a) and initial concentration (b) on photooxidation of γ-oryzanol at room temperature.
Figure 4
Figure 4
Effect of β-carotene (0–12 ppm) (a) and γ–oryzanol (0–400 ppm) (b) on photooxidation of 0.03 M linoleic acid containing erythrosine sensitizer (25 ppm) in ethanol under light 3400 lux up to 4 h at room temperature.
Figure 5
Figure 5
The plot of [AO2]−1 or [lipid hydroperoxide]−1vs. [A]−1 or [linoleic acid]−1.
Figure 6
Figure 6
The plot of slope/intercept from Figure 5 vs. the concentration of γ-oryzanol.
Figure 7
Figure 7
The decreasing rate of γ-oryzanol and linoleic acid during photo-oxidation at 3400 lux.
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