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Review
. 2021 May-Jun;11(3):224-237.

Antiglycation and antitumoral activity of Tribulus terrestris dry extract

Célia Cristina Malaguti Figueiredo  1 , Amanda Costa Gomes  1 , Filipe Oliveira Granero  1 , João Luiz Bronzel Junior  1 , Luciana Pereira Silva  2 , Ana Lúcia Tasca Gois Ruiz  3 , Regildo Márcio Gonçalves da Silva  1   4
Affiliations
Review

Antiglycation and antitumoral activity of Tribulus terrestris dry extract

Célia Cristina Malaguti Figueiredo et al. Avicenna J Phytomed. 2021 May-Jun.

Abstract

Objective: Investigation of the antiglycation and antitumoral potential of standardized and saponins-enriched extracts of Tribulus terrestris herbal medicine.

Materials and methods: The procedures for the evaluation of the antiglycation activity of the standardized (TtSE) and saponins-enriched (TtEE) extracts of T. terrestris were: determination of relative mobility in electrophoresis (RME), free amino groups using OPA method and advanced glycation end-products (AGEs) fluorescence. Antioxidant activity was determined by DPPH radical scavenging test. In vitro antitumor activity of TtSE and TtEE was evaluated in human tumor cell lines.

Results: The results were obtained by antiglycation tests (RME, OPA method and AGEs fluorescence determination), using BSA as protein and ribose as glycation agent, and antioxidant assay (DPPH test); it was verified that both extracts of T. terrestris have antiglycation and antioxidant activity. In addition, the extracts were able to induce death of more than 50% of human tumor cell lines.

Conclusion: The present study showed that standardized and saponins-enriched extracts of T. terrestris herbal medicine present antiglycation and antioxidant and antiproliferative action in human tumor cells lines. The saponins-enriched extract proved a greater antiglycation and antioxidant activity in comparison to the standardized type.

Keywords: Antiproliferative; Protein glycation; Steroidal saponins; Tribulus terrestris.

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Figures

Figure 1
Figure 1
HPLC chromatogram of saponins-enriched extract of T. terrestris herbal medicine. Peak identification: GITO=gitogenin; PROT=protodioscin and DIOS=diosgenin
Figure 2
Figure 2
Relative mobility in electrophoresis test for evaluation of antiglycation capacity by system A=BSA; 1=BSA+Ribose; 2=BSA+Ribose+AMG; 3=BSA+Ribose+TtSE; 4=BSA+Ribose+TtEE and 5=BSA+Ribose+Diosgenin. Where BSA=bovine serum albumin, AMG=Aminoguanidine, TtSE=T. terrestris standard extract and TtEE=T. terrestris saponins-enriched extract
Figure 3
Figure 3
Percentage of free amino groups determined by OPA method with the TtSE (T. terrestris standard extract), TtEE (T. terrestris saponins-enriched extract) and DIOS (saponin diosgenin) in association with BSA (Bovine Serum Albumin) as protein and RIB (Ribose) as glycation agent. Different letters superscripts indicated mean difference between treatments significant at p<0.05
Figure 4
Figure 4
Percentage of glycation inhibition: TtSE (T. terrestris standard extract); TrEE (T. terrestris saponins-enriched extract); AMG (Aminoguanidine), BSA (Bovine Serum Albumin) and RIB (Ribose).Different letters superscripts indicated mean difference between treatments significant at p<0.05
Figure 5
Figure 5
AGEs (Advanced Glycation End-Products) formation inhibition values determined by AGEs fluorescence: TtSE (T. terrestris standard extract); TrEE (T. terrestris saponins-enriched extract); AMG (Aminoguanidine), BSA (Bovine Serum Albumin) and RIB (Ribose). Different letters superscripts indicated mean difference between treatments significant at p<0.05
Figure 6
Figure 6
In vitro antiproliferative activity of A=TtSE (T. terrestris standard extract); B=TrEE (T. terrestris saponins-enriched extract) in human tumor cell lines: U25 (glioma), MCF-7 (breast), NCI-ADR/RES (ovary with multidrug resistant phenotypes), 786-O (kidney), NCI-H460 (lung), PC-3 (prostate), OVCAR-03 (ovary), HT-29 (colon) and human non-tumor lineage: q=HaCaT (keratinocyte). Doxorubicin (C) was used as a reference standard
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