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. 2021:2295:351-361.
doi: 10.1007/978-1-0716-1362-7_19.

Collection and Analysis of Phloem Lipids

Susanne Hoffmann-Benning  1
Affiliations

Collection and Analysis of Phloem Lipids

Susanne Hoffmann-Benning. Methods Mol Biol. 2021.

Abstract

The plant phloem is a long-distance conduit for the transport of assimilates but also of mobile developmental and stress signals. These signals can be sugars, metabolites, amino acids, peptides, proteins, microRNA, or mRNA. Yet small lipophilic molecules such as oxylipins and, more recently, phospholipids have emerged as possible long-distance signals as well. Analysis of phloem (phospho)lipids, however, requires enrichment, purification, and sensitive analysis. This chapter describes the EDTA-facilitated approach of phloem exudate collection, phase partitioning against chloroform-methanol for lipid separation and enrichment, and analysis/identification of phloem lipids using LC-MS with multiplexed collision induced dissociation (CID).

Keywords: Lipids; Liquid chromatography–mass spectrometry (LC-MS); Long-distance lipid-mediated signaling; Multiplexed collision induced dissociation (CID); Phloem.

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