Synthetic biology approaches to actinomycete strain improvement

Rainer Breitling¹, Martina Avbelj², Oksana Bilyk¹, Francesco Del Carratore¹, Alessandro Filisetti³, Erik K R Hanko¹, Marianna Iorio⁴, Rosario Pérez Redondo⁵, Fernando Reyes⁶, Michelle Rudden⁷, Emmanuele Severi⁷, Lucija Slemc², Kamila Schmidt¹, Dominic R Whittall¹, Stefano Donadio⁴, Antonio Rodríguez García⁵, Olga Genilloud⁶, Gregor Kosec⁸, Davide De Lucrezia³, Hrvoje Petković², Gavin Thomas⁷, Eriko Takano¹

Affiliations

¹ Department of Chemistry, Manchester Institute of Biotechnology, Manchester Synthetic Biology Research Centre SYNBIOCHEM, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
² Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
³ Explora Biotech Srl, Doulix business unit, Via Torino 107, 30133 Venice, Italy.
⁴ Naicons srl., Viale Ortles 22/4, 20139 Milano, Italy.
⁵ INBIOTEC Instituto de Biotecnología de León, Avenida Real 1, 24006 León, Spain.
⁶ Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avenida del Conocimiento 34, Parque Tecnologico de Ciencias de la Salud, 18016 Armilla, Granada, Spain.
⁷ Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK.
⁸ Acies Bio d.o.o., Tehnološki Park 21, 1000, Ljubljana, Slovenia.

PMID: 34057181
PMCID: PMC8195692
DOI: 10.1093/femsle/fnab060

Review

Synthetic biology approaches to actinomycete strain improvement

Rainer Breitling et al. FEMS Microbiol Lett. 2021.

. 2021 Jun 11;368(10):fnab060.

doi: 10.1093/femsle/fnab060.

Authors

Affiliations

¹ Department of Chemistry, Manchester Institute of Biotechnology, Manchester Synthetic Biology Research Centre SYNBIOCHEM, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
² Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
³ Explora Biotech Srl, Doulix business unit, Via Torino 107, 30133 Venice, Italy.
⁴ Naicons srl., Viale Ortles 22/4, 20139 Milano, Italy.
⁵ INBIOTEC Instituto de Biotecnología de León, Avenida Real 1, 24006 León, Spain.
⁶ Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avenida del Conocimiento 34, Parque Tecnologico de Ciencias de la Salud, 18016 Armilla, Granada, Spain.
⁷ Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK.
⁸ Acies Bio d.o.o., Tehnološki Park 21, 1000, Ljubljana, Slovenia.

PMID: 34057181
PMCID: PMC8195692
DOI: 10.1093/femsle/fnab060

Abstract

Their biochemical versatility and biotechnological importance make actinomycete bacteria attractive targets for ambitious genetic engineering using the toolkit of synthetic biology. But their complex biology also poses unique challenges. This mini review discusses some of the recent advances in synthetic biology approaches from an actinomycete perspective and presents examples of their application to the rational improvement of industrially relevant strains.

Keywords: actinobacteria; actinomycete; biosynthesis; biotechnology; synthetic biology.

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Synthetic biology approaches to actinomycete strain improvement

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Synthetic biology approaches to actinomycete strain improvement

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