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. 2021:2308:163-176.
doi: 10.1007/978-1-0716-1425-9_14.

Imaging of Bone Marrow Plasma Cells and of Their Niches

Carolin Ulbricht  1   2 , Raluca A Niesner  2   3 , Anja E Hauser  4   5
Affiliations

Imaging of Bone Marrow Plasma Cells and of Their Niches

Carolin Ulbricht et al. Methods Mol Biol. 2021.

Abstract

Decade-long survival of plasma cells in the bone marrow has long been a puzzling matter. To understand how plasma cells are maintained and supported by survival-niches to account for lifelong antibody production demands new intravital imaging techniques that are able to follow up a single cell and their interaction with other cell types in situ. We achieved to successfully establish longitudinal imaging of the bone marrow (LIMB) that is based on an implantable endoscopic device. In this chapter, basic approaches on how to investigate plasma cell-stroma interaction and surgical implantation procedures are introduced.

Keywords: Bone marrow; Intravital imaging, Fluorescent reporter mice; Plasma cells; Survival niches; Two-photon microscopy.

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References

    1. Lemke A, Kraft M, Roth K et al (2016) Long-lived plasma cells are generated in mucosal immune responses and contribute to the bone marrow plasma cell pool in mice. Mucosal Immunol 9:83–97. https://doi.org/10.1038/mi.2015.38 - DOI - PubMed
    1. Manz RA, Thiel A, Radbruch A (1997) Lifetime of plasma cells in the bone marrow. Nature 388:133–134. https://doi.org/10.1038/40540 - DOI - PubMed
    1. Slifka MK, Antia R, Whitmire JK, Ahmed R (1998) Humoral immunity due to long-lived plasma cells. Immunity 8:363–372. https://doi.org/10.1016/S1074-7613(00)80541-5 - DOI - PubMed
    1. Holzwarth K, Köhler R, Philipsen L et al (2018) Multiplexed fluorescence microscopy reveals heterogeneity among stromal cells in mouse bone marrow sections. Cytom A 93:876–888. https://doi.org/10.1002/cyto.a.23526 - DOI
    1. Zehentmeier S, Roth K, Cseresnyes Z et al (2014) Static and dynamic components synergize to form a stable survival niche for bone marrow plasma cells. Eur J Immunol 44:2306–2317. https://doi.org/10.1002/eji.201344313 - DOI - PubMed

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