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. 2021 Jul;18(7):1826-1828.
doi: 10.1038/s41423-021-00700-0. Epub 2021 May 31.

Cross-reactive antibody immunity against SARS-CoV-2 in children and adults

Elizabeth Fraley  1 , Cas LeMaster  1 , Dithi Banerjee  2 , Santosh Khanal  1 , Rangaraj Selvarangan  3   4 , Todd Bradley  5   6   7   8
Affiliations

Cross-reactive antibody immunity against SARS-CoV-2 in children and adults

Elizabeth Fraley et al. Cell Mol Immunol. 2021 Jul.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1. Antibody responses to SARS-CoV-2 in previously infected and healthy populations.
(A) Multiplex bead-based antibody binding assay to measure the IgG antibody response to four SARS-CoV-2 viral antigens (S1, S2, RBD, and NP). The median fluorescence intensity (MFI) is shown, and background well subtraction was used to remove nonspecific signals. Each dot represents an individual in three groups: SARS-CoV-2 convalescent adults (left, n = 44), adults with no prior SARS-CoV-2 infection (middle, n = 44), and pediatric individuals with no prior SARS-CoV-2 (right, n = 866). Bars represent the group median. The dashed line indicates a threshold determined by the sum of the mean and standard deviation for the negative control (beads without antigen). The numbers above each antigen are the numbers of individuals above the determined threshold. Plasma IgG binding to SARS-CoV-2 peptides spanning the SARS-CoV-2 spike protein ((B) S1 and (C) S2 subunits; 12-mer overlapping peptides). Seropositive (top) represents 14 individuals previously infected with SARS-CoV-2 (I1–I14), and seronegative (bottom) represents seven children (C1–C7) and seven adults (A1–A7) recruited prior to the COVID-19 pandemic. Each peptide was printed in triplicate, and the Log2 of the mean fluorescent intensity (F635) for each peptide was graphed. The row color corresponds to the minimum and maximum intensity for all peptides for each individual. Known regions of the spike protein are annotated above the heatmaps. Line graphs display the mean group Z-score for each peptide in S1 and S2 from the seronegative group (green; n = 14) and seropositive group (red; n = 14)
See this image and copyright information in PMC

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